Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 146193..146718 Replicon plasmid unnamed3
Accession NZ_CP083875
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag LCE27_RS24060 Protein ID WP_001159868.1
Coordinates 146193..146498 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LCE27_RS24065 Protein ID WP_000813634.1
Coordinates 146500..146718 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS24045 (142103) 142103..143269 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
LCE27_RS24050 (143857) 143857..144612 - 756 WP_000852146.1 replication initiation protein RepE -
LCE27_RS24055 (145386) 145386..146192 - 807 WP_000016982.1 site-specific integrase -
LCE27_RS24060 (146193) 146193..146498 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
LCE27_RS24065 (146500) 146500..146718 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LCE27_RS24070 (147426) 147426..148421 + 996 WP_000246636.1 hypothetical protein -
LCE27_RS24075 (148425) 148425..149357 + 933 WP_058682609.1 S-4TM family putative pore-forming effector -
LCE27_RS24090 (151231) 151231..151389 + 159 WP_228955855.1 abortive infection family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / dfrA12 / aadA2 / qacE / sul1 - 1..170625 170625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T217654 WP_001159868.1 NZ_CP083875:c146498-146193 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217654 NZ_CP114551:c1012031-1011756 [Xylella fastidiosa subsp. pauca]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTGGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGACGAGAATGCTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT217654 WP_000813634.1 NZ_CP083875:c146718-146500 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217654 NZ_CP114551:c1011703-1011533 [Xylella fastidiosa subsp. pauca]
ATGCCAATTTCATCTCCGTTATCACGTACCAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGTGGTACGAATTCC
CGTTGAATTCGAGCTGCCGGGTGATCGTGTTCTGATCCACCGTGAGGAGGACAAGCTCATTATTGAGCCGATCACCCGGC
CAACCAACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References