Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4226470..4226967 Replicon chromosome
Accession NZ_CP083872
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCE27_RS20520 Protein ID WP_001173343.1
Coordinates 4226470..4226643 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LCE27_RS20525 Protein ID WP_000398619.1
Coordinates 4226671..4226967 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS20510 (4223140) 4223140..4224597 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LCE27_RS20515 (4224834) 4224834..4226351 + 1518 WP_001295074.1 lysine--tRNA ligase -
LCE27_RS20520 (4226470) 4226470..4226643 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCE27_RS20525 (4226671) 4226671..4226967 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCE27_RS20530 (4227194) 4227194..4227466 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LCE27_RS20535 (4227478) 4227478..4227708 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LCE27_RS20540 (4227889) 4227889..4229520 + 1632 WP_001216477.1 sensor histidine kinase -
LCE27_RS20545 (4229517) 4229517..4230236 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T217648 WP_001173343.1 NZ_CP083872:c4226643-4226470 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T217648 NZ_CP114550:c1413595-1413314 [Xylella fastidiosa subsp. pauca]
GTGCGGGAAATTAAACAACTCGGGCAGTTCAAACGTGACTTGAAGCGCGAAGCCAAGGGGCAGCATCGCGCCACGCTGGA
AGACGACCTGCTGCCCGTGATCGATGCCCTTGCTAAAGACATGCCCCTTGAGCCTCGGCACAGAGATCACGCACTGCTTG
GCAACTGGAAAGACCTTCGTGATTGTCACATCAAGCTCGACCTAGTCCTGATCTACACGCGGGTGGATAGCAAAACGCTG
ACGCTCGTCCGTCTCGGCTCCCATGCGGAACTCGGGCTGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT217648 WP_000398619.1 NZ_CP083872:c4226967-4226671 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217648 NZ_CP114550:c1413842-1413582 [Xylella fastidiosa subsp. pauca]
ATGGCTGCGAATCAGCTTGTGCAAGCGCGCATCGATGGTGCGATCAAGGCAGAGGCAACCGTGGTACTGGCGGCGATGGG
GTTGACCATTTCCGATGCGGTTCGGCTGTTGCTCACGAAGGTGGCTCAGGACAAGGCGTTGCCTTTTGAGCCGCTAATCC
CTAATGCCACCACCATCAAGGCCATGCGGGAGGCTCGTGCAGGTAAGGGCGAGACCGTCACGCTGGGTGAACTTCGAGCG
ACTATCCGTGCGGGAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References