Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4013404..4013980 Replicon chromosome
Accession NZ_CP083872
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LCE27_RS19410 Protein ID WP_001297643.1
Coordinates 4013693..4013980 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LCE27_RS19405 Protein ID WP_000063160.1
Coordinates 4013404..4013706 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS19390 (4010000) 4010000..4012150 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LCE27_RS19395 (4012200) 4012200..4012403 + 204 WP_000467859.1 YbdD/YjiX family protein -
LCE27_RS19400 (4012414) 4012414..4013370 + 957 WP_001297640.1 GTPase -
LCE27_RS19405 (4013404) 4013404..4013706 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LCE27_RS19410 (4013693) 4013693..4013980 - 288 WP_001297643.1 BrnT family toxin Toxin
LCE27_RS19415 (4014179) 4014179..4014875 - 697 Protein_3792 IS1 family transposase -
LCE27_RS19420 (4015190) 4015190..4016422 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LCE27_RS19425 (4016463) 4016463..4017743 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4014179..4014388 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T217646 WP_001297643.1 NZ_CP083872:c4013980-4013693 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T217646 NZ_CP114550:c1221460-1221158 [Xylella fastidiosa subsp. pauca]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTTGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTGCTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATGAGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT217646 WP_000063160.1 NZ_CP083872:c4013706-4013404 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT217646 NZ_CP114550:c1221161-1220856 [Xylella fastidiosa subsp. pauca]
ATGAATAATGAGACGTTTAGTCGATACGACACCGCTGACTACCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGTATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAGGCGCTTGGTTTACGGATGTCATTCACTCCTTCCTCTATGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References