Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3416080..3416698 Replicon chromosome
Accession NZ_CP083872
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCE27_RS16520 Protein ID WP_001291435.1
Coordinates 3416480..3416698 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCE27_RS16515 Protein ID WP_000344800.1
Coordinates 3416080..3416454 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS16505 (3411169) 3411169..3412362 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCE27_RS16510 (3412385) 3412385..3415534 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCE27_RS16515 (3416080) 3416080..3416454 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCE27_RS16520 (3416480) 3416480..3416698 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LCE27_RS16525 (3416870) 3416870..3417421 + 552 WP_000102564.1 maltose O-acetyltransferase -
LCE27_RS16530 (3417537) 3417537..3418007 + 471 WP_000136192.1 YlaC family protein -
LCE27_RS16535 (3418171) 3418171..3419721 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCE27_RS16540 (3419763) 3419763..3420116 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LCE27_RS16550 (3420495) 3420495..3420806 + 312 WP_000409911.1 MGMT family protein -
LCE27_RS16555 (3420837) 3420837..3421409 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217643 WP_001291435.1 NZ_CP083872:3416480-3416698 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T217643 NZ_CP114550:c1217623-1217348 [Xylella fastidiosa subsp. pauca]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTGGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGACGAGAATGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT217643 WP_000344800.1 NZ_CP083872:3416080-3416454 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT217643 NZ_CP114550:c1217295-1217125 [Xylella fastidiosa subsp. pauca]
ATGCCAATTTCATCTCCGTTATCACGTACCAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGTGGTACGAATTCC
CGTTGAATTCGAGCTGCCGGGTGATCGTGTTCTGATCCACCGTGAGGAGGACAAGCTCATTATTGAGCCGATCACCCGGC
CAACCAACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References