Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4040134..4040710 Replicon chromosome
Accession NZ_CP083869
Organism Escherichia coli strain NDM6

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LCE24_RS20880 Protein ID WP_001297643.1
Coordinates 4040423..4040710 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LCE24_RS20875 Protein ID WP_000063160.1
Coordinates 4040134..4040436 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE24_RS20860 (4036730) 4036730..4038880 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LCE24_RS20865 (4038930) 4038930..4039133 + 204 WP_000467859.1 YbdD/YjiX family protein -
LCE24_RS20870 (4039144) 4039144..4040100 + 957 WP_001297640.1 GTPase -
LCE24_RS20875 (4040134) 4040134..4040436 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LCE24_RS20880 (4040423) 4040423..4040710 - 288 WP_001297643.1 BrnT family toxin Toxin
LCE24_RS20885 (4040909) 4040909..4041605 - 697 Protein_3815 IS1 family transposase -
LCE24_RS20890 (4041920) 4041920..4043152 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LCE24_RS20895 (4043193) 4043193..4044473 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4040909..4041118 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T217624 WP_001297643.1 NZ_CP083869:c4040710-4040423 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T217624 NZ_CP114546:29008-29259 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT217624 WP_000063160.1 NZ_CP083869:c4040436-4040134 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT217624 NZ_CP114546:28749-29015 [Ligilactobacillus salivarius]
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References