Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 860322..860976 Replicon chromosome
Accession NZ_CP083869
Organism Escherichia coli strain NDM6

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LCE24_RS05560 Protein ID WP_000244781.1
Coordinates 860569..860976 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCE24_RS05555 Protein ID WP_000354046.1
Coordinates 860322..860588 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE24_RS05535 (856410) 856410..857843 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
LCE24_RS05540 (857888) 857888..858199 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
LCE24_RS05545 (858363) 858363..859022 + 660 WP_000250269.1 hemolysin III family protein -
LCE24_RS05550 (859099) 859099..860079 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LCE24_RS05555 (860322) 860322..860588 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCE24_RS05560 (860569) 860569..860976 + 408 WP_000244781.1 protein YgfX Toxin
LCE24_RS05565 (861016) 861016..861537 - 522 WP_001521233.1 flavodoxin FldB -
LCE24_RS05570 (861649) 861649..862545 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LCE24_RS05575 (862570) 862570..863280 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCE24_RS05580 (863286) 863286..865019 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T217608 WP_000244781.1 NZ_CP083869:860569-860976 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T217608 NZ_CP114518:c132155-131904 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT217608 WP_000354046.1 NZ_CP083869:860322-860588 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT217608 NZ_CP114518:c132414-132148 [Ligilactobacillus salivarius]
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References