Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 62748..63307 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP083867 | ||
| Organism | Escherichia coli strain NDM6 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0SVC8 |
| Locus tag | LCE24_RS00370 | Protein ID | WP_000421272.1 |
| Coordinates | 63032..63307 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | LCE24_RS00365 | Protein ID | WP_001387467.1 |
| Coordinates | 62748..63032 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCE24_RS00335 (58216) | 58216..58563 | - | 348 | WP_000679427.1 | quaternary ammonium compound efflux SMR transporter QacE delta 1 | - |
| LCE24_RS00340 (58680) | 58680..59518 | - | 839 | Protein_67 | AadA family aminoglycoside 3''-O-nucleotidyltransferase | - |
| LCE24_RS00345 (59524) | 59524..59814 | - | 291 | WP_001336345.1 | DUF1010 domain-containing protein | - |
| LCE24_RS00350 (59926) | 59926..60423 | - | 498 | WP_001083725.1 | trimethoprim-resistant dihydrofolate reductase DfrA12 | - |
| LCE24_RS00355 (60568) | 60568..61434 | + | 867 | Protein_70 | class 1 integron integrase IntI1 | - |
| LCE24_RS00360 (61489) | 61489..62193 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| LCE24_RS00365 (62748) | 62748..63032 | + | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| LCE24_RS00370 (63032) | 63032..63307 | + | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LCE24_RS00380 (64190) | 64190..64393 | + | 204 | Protein_75 | hypothetical protein | - |
| LCE24_RS00385 (64518) | 64518..64646 | - | 129 | Protein_76 | IS481 family transposase | - |
| LCE24_RS00390 (64679) | 64679..65848 | - | 1170 | WP_001072355.1 | hypothetical protein | - |
| LCE24_RS00395 (66380) | 66380..66648 | + | 269 | Protein_78 | type II toxin-antitoxin system ParD family antitoxin | - |
| LCE24_RS00400 (66635) | 66635..66924 | + | 290 | Protein_79 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LCE24_RS00405 (66991) | 66991..67173 | + | 183 | WP_042344623.1 | hypothetical protein | - |
| LCE24_RS00410 (67294) | 67294..68034 | + | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / aadA2 | - | 1..224320 | 224320 | |
| - | inside | IScluster/Tn | tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / aadA2 | - | 4113..63800 | 59687 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 10.0871
>T217600 WP_000421272.1 NZ_CP083867:63032-63307 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T217600 NZ_CP114508:c34183-33932 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT217600 WP_001387467.1 NZ_CP083867:62748-63032 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT217600 NZ_CP114508:c34442-34176 [Ligilactobacillus salivarius]
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SVC8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |