Detailed information of TA system TA217585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1722636..1723433
• Replicon: chromosome
• Accession: NZ_CP083862
• Organism: Enterobacter kobei strain 11743-yvys

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LCD49_RS08195
• Protein ID: WP_176450254.1
• Coordinates: 1722909..1723433 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: LCD49_RS08190
• Protein ID: WP_014883303.1
• Coordinates: 1722636..1722905 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD49_RS08160 (LCD49_08170)	1718257..1718757	+	501	WP_014883299.1	DedA family protein	-
LCD49_RS08165 (LCD49_08175)	1718861..1720102	+	1242	WP_249050415.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
LCD49_RS08170 (LCD49_08180)	1720136..1720363	-	228	WP_014883300.1	YccJ family protein	-
LCD49_RS08175 (LCD49_08185)	1720382..1720978	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
LCD49_RS08180 (LCD49_08190)	1721370..1721540	+	171	WP_023332656.1	general stress protein	-
LCD49_RS08185 (LCD49_08195)	1721657..1722574	+	918	WP_047028038.1	DMT family transporter	-
LCD49_RS08190 (LCD49_08200)	1722636..1722905	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
LCD49_RS08195 (LCD49_08205)	1722909..1723433	+	525	WP_176450254.1	GNAT family N-acetyltransferase	Toxin
LCD49_RS08200 (LCD49_08210)	1723507..1724829	-	1323	WP_045281263.1	pyrimidine utilization transport protein G	-
LCD49_RS08205 (LCD49_08215)	1724851..1725345	-	495	WP_045281262.1	pyrimidine utilization flavin reductase protein F	-
LCD49_RS08210 (LCD49_08220)	1725355..1725945	-	591	WP_045281261.1	malonic semialdehyde reductase	-
LCD49_RS08215 (LCD49_08225)	1725955..1726755	-	801	WP_023338236.1	pyrimidine utilization protein D	-
LCD49_RS08220 (LCD49_08230)	1726763..1727149	-	387	WP_045281260.1	pyrimidine utilization protein C	-
LCD49_RS08225 (LCD49_08235)	1727161..1727850	-	690	WP_014883310.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19689.59 Da
• Isoelectric Point: 8.9464

>T217585 WP_176450254.1 NZ_CP083862:1722909-1723433 [Enterobacter kobei]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T217585 NZ_CP114375:4084882-4085100 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT217585 WP_014883303.1 NZ_CP083862:1722636-1722905 [Enterobacter kobei]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT217585 NZ_CP114375:4084481-4084855 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTAGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3