Detailed information of TA system TA217582

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 537313..538016
• Replicon: chromosome
• Accession: NZ_CP083862
• Organism: Enterobacter kobei strain 11743-yvys

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LCD49_RS02550
• Protein ID: WP_063308406.1
• Coordinates: 537675..538016 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LCD49_RS02545
• Protein ID: WP_063308407.1
• Coordinates: 537313..537654 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD49_RS02510 (LCD49_02515)	532464..533339	+	876	WP_063308414.1	GTPase family protein	-
LCD49_RS02515 (LCD49_02520)	533583..534299	+	717	WP_063308413.1	WYL domain-containing protein	-
LCD49_RS02520 (LCD49_02525)	534335..534787	+	453	WP_082826293.1	hypothetical protein	-
LCD49_RS02525 (LCD49_02530)	534859..535332	+	474	WP_063308411.1	hypothetical protein	-
LCD49_RS02530 (LCD49_02535)	535452..536273	+	822	WP_063308410.1	DUF932 domain-containing protein	-
LCD49_RS02535 (LCD49_02540)	536304..536747	+	444	WP_063308409.1	antirestriction protein	-
LCD49_RS02540 (LCD49_02545)	536760..537302	+	543	WP_063308408.1	DNA repair protein RadC	-
LCD49_RS02545 (LCD49_02550)	537313..537654	+	342	WP_063308407.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LCD49_RS02550 (LCD49_02555)	537675..538016	+	342	WP_063308406.1	TA system toxin CbtA family protein	Toxin
LCD49_RS02555 (LCD49_02560)	538275..539282	+	1008	WP_017880110.1	restriction endonuclease	-
LCD49_RS02560 (LCD49_02565)	539428..540372	-	945	WP_063308405.1	tryptophan--tRNA ligase	-
LCD49_RS02565 (LCD49_02570)	540572..540997	+	426	WP_045134374.1	FosA/FosA2 family fosfomycin resistance glutathione transferase	-
LCD49_RS02570 (LCD49_02575)	540983..541990	-	1008	WP_045134373.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	513392..543264	29872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12777.76 Da
• Isoelectric Point: 8.9919

>T217582 WP_063308406.1 NZ_CP083862:537675-538016 [Enterobacter kobei]
MKTLPATTPQAAKLCLSPVTVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWEEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T217582 NZ_CP114375:815213-815623 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12722.34 Da
• Isoelectric Point: 6.4739

>AT217582 WP_063308407.1 NZ_CP083862:537313-537654 [Enterobacter kobei]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT217582 NZ_CP114375:814966-815232 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA