Detailed information of TA system TA217548

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 567723..568402
• Replicon: chromosome
• Accession: NZ_CP083853
• Organism: Enterobacter roggenkampii strain 12795-yvys

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LCD45_RS02705
• Protein ID: WP_208558524.1
• Coordinates: 568061..568402 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LCD45_RS02700
• Protein ID: WP_208558525.1
• Coordinates: 567723..568040 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD45_RS02660 (LCD45_02675)	562938..563762	+	825	WP_208558530.1	DUF932 domain-containing protein	-
LCD45_RS02665 (LCD45_02680)	563971..564684	+	714	WP_208558529.1	DeoR family transcriptional regulator	-
LCD45_RS02670 (LCD45_02685)	564973..565839	+	867	WP_208558528.1	hypothetical protein	-
LCD45_RS02675 (LCD45_02690)	565934..566167	+	234	WP_105628388.1	DUF905 family protein	-
LCD45_RS02680 (LCD45_02695)	566273..566731	+	459	WP_105628387.1	antirestriction protein	-
LCD45_RS02685 (LCD45_02700)	566741..566959	+	219	WP_090083248.1	hypothetical protein	-
LCD45_RS02690 (LCD45_02705)	567002..567481	+	480	WP_208558527.1	DNA repair protein RadC	-
LCD45_RS02695 (LCD45_02710)	567490..567705	+	216	WP_208558526.1	DUF987 family protein	-
LCD45_RS02700 (LCD45_02715)	567723..568040	+	318	WP_208558525.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LCD45_RS02705 (LCD45_02720)	568061..568402	+	342	WP_208558524.1	TA system toxin CbtA family protein	Toxin
LCD45_RS02710 (LCD45_02725)	568519..569352	+	834	WP_208558523.1	DUF4942 domain-containing protein	-
LCD45_RS02715 (LCD45_02730)	569887..573195	-	3309	WP_208558522.1	AAA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	542208..575959	33751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13032.88 Da
• Isoelectric Point: 8.4753

>T217548 WP_208558524.1 NZ_CP083853:568061-568402 [Enterobacter roggenkampii]
MKLQPATTSRAVKPYLSPVTVWQMLLTRLFEKHYGLTLNDTPFSDENVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAEDILRARQATGLLQQSRNNAVR

Nucleotide

• Length: 342 bp

>T217548 NZ_CP114355:67656-68138 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11880.48 Da
• Isoelectric Point: 6.6283

>AT217548 WP_208558525.1 NZ_CP083853:567723-568040 [Enterobacter roggenkampii]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCVILYH
NDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT217548 NZ_CP114355:67402-67668 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA