Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1990193..1990813 Replicon chromosome
Accession NZ_CP083849
Organism Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LCD40_RS09825 Protein ID WP_015571250.1
Coordinates 1990595..1990813 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LCD40_RS09820 Protein ID WP_006809850.1
Coordinates 1990193..1990567 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD40_RS09810 (LCD40_09825) 1985320..1986513 + 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCD40_RS09815 (LCD40_09830) 1986536..1989682 + 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
LCD40_RS09820 (LCD40_09835) 1990193..1990567 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LCD40_RS09825 (LCD40_09840) 1990595..1990813 + 219 WP_015571250.1 HHA domain-containing protein Toxin
LCD40_RS09830 (LCD40_09845) 1991022..1991573 + 552 WP_023303203.1 maltose O-acetyltransferase -
LCD40_RS09835 (LCD40_09850) 1991690..1992157 + 468 WP_017383209.1 YlaC family protein -
LCD40_RS09840 (LCD40_09855) 1992129..1993589 - 1461 WP_023303202.1 PLP-dependent aminotransferase family protein -
LCD40_RS09845 (LCD40_09860) 1993691..1994401 + 711 WP_023303201.1 GNAT family protein -
LCD40_RS09850 (LCD40_09865) 1994398..1994538 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD40_RS09855 (LCD40_09870) 1994541..1994801 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T217538 WP_015571250.1 NZ_CP083849:1990595-1990813 [Enterobacter hormaechei subsp. steigerwaltii]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T217538 NZ_CP114354:368555-368902 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGAGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT217538 WP_006809850.1 NZ_CP083849:1990193-1990567 [Enterobacter hormaechei subsp. steigerwaltii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT217538 NZ_CP114354:368902-369201 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References