Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 60025..61648 Replicon plasmid p16773_1
Accession NZ_CP083843
Organism Enterobacter asburiae strain 16773-yvys

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LCD43_RS23295 Protein ID WP_000088044.1
Coordinates 60323..61648 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LCD43_RS23290 Protein ID WP_012477377.1
Coordinates 60025..60318 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD43_RS23270 (LCD43_23295) 55824..56216 + 393 WP_000341066.1 hypothetical protein -
LCD43_RS23275 (LCD43_23300) 56875..57531 + 657 WP_000462754.1 trypsin-like serine protease -
LCD43_RS23280 (LCD43_23305) 57734..58231 + 498 WP_000340139.1 membrane protein -
LCD43_RS23285 (LCD43_23310) 58236..59624 + 1389 WP_012695445.1 hypothetical protein -
LCD43_RS23290 (LCD43_23315) 60025..60318 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LCD43_RS23295 (LCD43_23320) 60323..61648 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LCD43_RS23300 (LCD43_23325) 61709..61915 + 207 WP_000134171.1 hypothetical protein -
LCD43_RS23305 (LCD43_23330) 62016..62426 + 411 WP_000985909.1 hypothetical protein -
LCD43_RS23310 (LCD43_23335) 62439..62963 + 525 WP_001805097.1 hypothetical protein -
LCD43_RS23315 (LCD43_23340) 63145..64147 - 1003 Protein_64 IS110-like element IS4321 family transposase -
LCD43_RS23320 (LCD43_23345) 64339..65042 - 704 Protein_65 IS6-like element IS26 family transposase -
LCD43_RS23325 (LCD43_23350) 65108..66044 + 937 Protein_66 transposase -
LCD43_RS23330 (LCD43_23355) 66080..66517 - 438 Protein_67 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / sul1 / blaDHA-1 / qnrB4 / qacE / ere(A) / aac(6')-IIc - 1..274805 274805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T217529 WP_000088044.1 NZ_CP083843:60323-61648 [Enterobacter asburiae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T217529 NZ_CP114332:c4165230-4164913 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT217529 WP_012477377.1 NZ_CP083843:60025-60318 [Enterobacter asburiae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT217529 NZ_CP114332:c4164920-4164633 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References