Detailed information of TA system TA217528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4667047..4667663
• Replicon: chromosome
• Accession: NZ_CP083842
• Organism: Enterobacter asburiae strain 16773-yvys

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCD43_RS22600
• Protein ID: WP_080298642.1
• Coordinates: 4667047..4667418 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: LCD43_RS22605
• Protein ID: WP_023309753.1
• Coordinates: 4667421..4667663 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD43_RS22585 (LCD43_22610)	4664547..4665449	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
LCD43_RS22590 (LCD43_22615)	4665446..4666081	+	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
LCD43_RS22595 (LCD43_22620)	4666078..4667007	+	930	WP_045404233.1	formate dehydrogenase accessory protein FdhE	-
LCD43_RS22600 (LCD43_22625)	4667047..4667418	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCD43_RS22605 (LCD43_22630)	4667421..4667663	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
LCD43_RS22610 (LCD43_22635)	4667863..4668783	+	921	WP_045404232.1	alpha/beta hydrolase	-
LCD43_RS22615 (LCD43_22640)	4668792..4669733	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
LCD43_RS22620 (LCD43_22645)	4669778..4670215	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
LCD43_RS22625 (LCD43_22650)	4670212..4671093	-	882	WP_023309756.1	virulence factor BrkB family protein	-
LCD43_RS22630 (LCD43_22655)	4671087..4671686	-	600	WP_045404231.1	glucose-1-phosphatase	-
LCD43_RS22635 (LCD43_22660)	4671807..4672607	-	801	WP_045404230.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T217528 WP_080298642.1 NZ_CP083842:c4667418-4667047 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T217528 NZ_CP114332:c2844371-2843043 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTCTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGTACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCTGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAATCTATGGT
CACTCCCGTTTTTGCAACACCGATTTTGACGACAAGTTGGCTTGCTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT217528 WP_023309753.1 NZ_CP083842:c4667663-4667421 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT217528 NZ_CP114332:c2844637-2844371 [Klebsiella variicola]
ATGACTTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0