Detailed information of TA system TA217525

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3544910..3545589
• Replicon: chromosome
• Accession: NZ_CP083842
• Organism: Enterobacter asburiae strain 16773-yvys

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LCD43_RS17175
• Protein ID: WP_045370410.1
• Coordinates: 3545323..3545589 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A9K1QZR3
• Locus tag: LCD43_RS17170
• Protein ID: WP_032642918.1
• Coordinates: 3544910..3545233 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD43_RS17140 (LCD43_17155)	3541088..3541357	+	270	WP_009653703.1	metal-sensing transcriptional repressor	-
LCD43_RS17145 (LCD43_17160)	3541370..3542512	+	1143	WP_029602872.1	nickel/cobalt efflux protein RcnA	-
LCD43_RS17150 (LCD43_17165)	3542673..3543113	+	441	WP_135695985.1	NirD/YgiW/YdeI family stress tolerance protein	-
LCD43_RS17155 (LCD43_17170)	3543334..3544155	+	822	WP_032642912.1	DUF932 domain-containing protein	-
LCD43_RS17160 (LCD43_17175)	3544188..3544658	+	471	WP_032642914.1	DNA repair protein RadC	-
LCD43_RS17165 (LCD43_17180)	3544667..3544888	+	222	WP_032642916.1	DUF987 domain-containing protein	-
LCD43_RS17170 (LCD43_17185)	3544910..3545233	+	324	WP_032642918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LCD43_RS17175 (LCD43_17190)	3545323..3545589	+	267	WP_045370410.1	TA system toxin CbtA family protein	Toxin
LCD43_RS17180 (LCD43_17195)	3546306..3546662	+	357	WP_023333137.1	hypothetical protein	-
LCD43_RS17185 (LCD43_17200)	3546750..3547094	+	345	WP_045404665.1	cupin domain-containing protein	-
LCD43_RS17190 (LCD43_17205)	3547163..3547720	+	558	WP_045404668.1	YceI family protein	-
LCD43_RS17195 (LCD43_17210)	3547712..3548503	-	792	WP_045404671.1	hypothetical protein	-
LCD43_RS17200 (LCD43_17215)	3548500..3549030	-	531	WP_080262529.1	RNA polymerase sigma factor	-
LCD43_RS17205 (LCD43_17220)	3549038..3549565	-	528	WP_045404673.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-87)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10206.66 Da
• Isoelectric Point: 5.5235

>T217525 WP_045370410.1 NZ_CP083842:3545323-3545589 [Enterobacter asburiae]
MVWQQLLTYLLEHHYGLTLNDTPFHDDTAIEEHIDAGITLADAVNFLVEKYELARIDRKGFTWREQPPFITATDILRAKK
NIGLLPRS

Nucleotide

• Length: 267 bp

>T217525 NZ_CP114332:1910563-1910895 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGACGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12082.82 Da
• Isoelectric Point: 6.6250

>AT217525 WP_032642918.1 NZ_CP083842:3544910-3545233 [Enterobacter asburiae]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGTFNEEEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLYHN
GLTCEADTLGSHGYVYIVIYPQAFATR

Nucleotide

• Length: 324 bp

>AT217525 NZ_CP114332:1910305-1910562 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA