Detailed information of TA system TA217512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 315483..316384
• Replicon: plasmid pAR0468_1
• Accession: NZ_CP083835
• Organism: Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: LCD48_RS25780
• Protein ID: WP_007372300.1
• Coordinates: 315483..315941 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LCD48_RS25785
• Protein ID: WP_008786588.1
• Coordinates: 315938..316384 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD48_RS25730 (LCD48_25730)	311131..311451	+	321	WP_233269684.1	hypothetical protein	-
LCD48_RS25735 (LCD48_25735)	311462..311860	+	399	WP_007372292.1	hypothetical protein	-
LCD48_RS25740 (LCD48_25740)	311944..312270	+	327	WP_007372293.1	hypothetical protein	-
LCD48_RS25745 (LCD48_25745)	312322..312537	+	216	WP_139156956.1	hypothetical protein	-
LCD48_RS25750 (LCD48_25750)	312633..312971	-	339	WP_007372294.1	hypothetical protein	-
LCD48_RS25755 (LCD48_25755)	313209..313652	+	444	WP_007372295.1	hypothetical protein	-
LCD48_RS25760 (LCD48_25760)	313771..314112	+	342	WP_007372296.1	hypothetical protein	-
LCD48_RS25765 (LCD48_25765)	314271..314825	+	555	WP_246917677.1	hypothetical protein	-
LCD48_RS25770 (LCD48_25770)	314875..315141	+	267	WP_007372298.1	hypothetical protein	-
LCD48_RS25775 (LCD48_25775)	315209..315439	-	231	WP_007372299.1	hypothetical protein	-
LCD48_RS25780 (LCD48_25780)	315483..315941	-	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
LCD48_RS25785 (LCD48_25785)	315938..316384	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
LCD48_RS25790 (LCD48_25790)	316533..316850	+	318	WP_007372302.1	hypothetical protein	-
LCD48_RS25795 (LCD48_25795)	316892..317734	+	843	WP_007372303.1	hypothetical protein	-
LCD48_RS25800 (LCD48_25800)	317706..317984	+	279	WP_007372304.1	hypothetical protein	-
LCD48_RS25805 (LCD48_25805)	318044..318487	+	444	WP_007372305.1	hypothetical protein	-
LCD48_RS25810 (LCD48_25810)	318774..318938	+	165	WP_007372306.1	hypothetical protein	-
LCD48_RS25815 (LCD48_25815)	319506..320348	+	843	WP_007372307.1	HNH endonuclease signature motif containing protein	-
LCD48_RS25820 (LCD48_25820)	320770..321021	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..373441	373441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T217512 WP_007372300.1 NZ_CP083835:c315941-315483 [Enterobacter asburiae]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T217512 NZ_CP114328:2040700-2040802 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT217512 WP_008786588.1 NZ_CP083835:c316384-315938 [Enterobacter asburiae]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT217512 NZ_CP114328:c2040808-2040664 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A837LJP8