Detailed information of TA system TA217495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1630937..1631734
• Replicon: chromosome
• Accession: NZ_CP083834
• Organism: Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LCD48_RS07795
• Protein ID: WP_172421424.1
• Coordinates: 1631234..1631734 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: LCD48_RS07790
• Protein ID: WP_021240811.1
• Coordinates: 1630937..1631206 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD48_RS07760 (LCD48_07760)	1626564..1627007	-	444	WP_039261676.1	helix-turn-helix domain-containing protein	-
LCD48_RS07765 (LCD48_07765)	1627160..1628401	+	1242	WP_039261681.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
LCD48_RS07770 (LCD48_07770)	1628435..1628662	-	228	WP_008499832.1	YccJ family protein	-
LCD48_RS07775 (LCD48_07775)	1628683..1629279	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
LCD48_RS07780 (LCD48_07780)	1629670..1629840	+	171	WP_023311077.1	general stress protein	-
LCD48_RS07785 (LCD48_07785)	1629958..1630875	+	918	WP_045888459.1	DMT family transporter	-
LCD48_RS07790 (LCD48_07790)	1630937..1631206	+	270	WP_021240811.1	DUF1778 domain-containing protein	Antitoxin
LCD48_RS07795 (LCD48_07795)	1631234..1631734	+	501	WP_172421424.1	GNAT family N-acetyltransferase	Toxin
LCD48_RS07800 (LCD48_07800)	1631808..1633130	-	1323	WP_045888458.1	pyrimidine utilization transport protein G	-
LCD48_RS07805 (LCD48_07805)	1633152..1633646	-	495	WP_023311082.1	pyrimidine utilization flavin reductase protein F	-
LCD48_RS07810 (LCD48_07810)	1633656..1634246	-	591	WP_039261690.1	malonic semialdehyde reductase	-
LCD48_RS07815 (LCD48_07815)	1634256..1635056	-	801	WP_032658084.1	pyrimidine utilization protein D	-
LCD48_RS07820 (LCD48_07820)	1635064..1635450	-	387	WP_010429508.1	pyrimidine utilization protein C	-
LCD48_RS07825 (LCD48_07825)	1635462..1636151	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18969.67 Da
• Isoelectric Point: 7.9145

>T217495 WP_172421424.1 NZ_CP083834:1631234-1631734 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T217495 NZ_CP114324:1910563-1910895 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGACGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10244.69 Da
• Isoelectric Point: 5.8836

>AT217495 WP_021240811.1 NZ_CP083834:1630937-1631206 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTIIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT217495 NZ_CP114324:1910305-1910562 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA