Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1055692..1056312 Replicon chromosome
Accession NZ_CP083834
Organism Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag LCD48_RS05060 Protein ID WP_008499287.1
Coordinates 1055692..1055910 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LCD48_RS05065 Protein ID WP_008499288.1
Coordinates 1055938..1056312 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD48_RS05030 (LCD48_05030) 1051706..1051966 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
LCD48_RS05035 (LCD48_05035) 1051969..1052109 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD48_RS05040 (LCD48_05040) 1052106..1052816 - 711 WP_045887942.1 GNAT family protein -
LCD48_RS05045 (LCD48_05045) 1052918..1054378 + 1461 WP_045887943.1 PLP-dependent aminotransferase family protein -
LCD48_RS05050 (LCD48_05050) 1054350..1054817 - 468 WP_023310598.1 YlaC family protein -
LCD48_RS05055 (LCD48_05055) 1054935..1055486 - 552 WP_045887944.1 maltose O-acetyltransferase -
LCD48_RS05060 (LCD48_05060) 1055692..1055910 - 219 WP_008499287.1 HHA domain-containing protein Toxin
LCD48_RS05065 (LCD48_05065) 1055938..1056312 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LCD48_RS05070 (LCD48_05070) 1056825..1059971 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit AcrB -
LCD48_RS05075 (LCD48_05075) 1059994..1061187 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T217494 WP_008499287.1 NZ_CP083834:c1055910-1055692 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T217494 NZ_CP114324:795335-795745 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTCCCCTGGCCGCTGAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGCACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT217494 WP_008499288.1 NZ_CP083834:c1056312-1055938 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT217494 NZ_CP114324:795088-795354 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References