Detailed information of TA system TA217493

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 573049..573724
• Replicon: chromosome
• Accession: NZ_CP083834
• Organism: Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LCD48_RS02750
• Protein ID: WP_192082478.1
• Coordinates: 573404..573724 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LCD48_RS02745
• Protein ID: WP_246917177.1
• Coordinates: 573049..573372 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD48_RS02720 (LCD48_02715)	568567..569547	-	981	WP_032430896.1	IS5-like element IS5 family transposase	-
LCD48_RS02725 (LCD48_02720)	569880..571172	+	1293	WP_246917167.1	hypothetical protein	-
LCD48_RS02730 (LCD48_02725)	571476..572294	+	819	WP_246917170.1	DUF932 domain-containing protein	-
LCD48_RS02735 (LCD48_02730)	572327..572797	+	471	WP_246917173.1	DNA repair protein RadC	-
LCD48_RS02740 (LCD48_02735)	572806..573027	+	222	WP_192082482.1	DUF987 family protein	-
LCD48_RS02745 (LCD48_02740)	573049..573372	+	324	WP_246917177.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LCD48_RS02750 (LCD48_02745)	573404..573724	+	321	WP_192082478.1	TA system toxin CbtA family protein	Toxin
LCD48_RS02755 (LCD48_02750)	573837..574667	+	831	WP_246917180.1	DUF4942 domain-containing protein	-
LCD48_RS02760 (LCD48_02755)	574744..575751	+	1008	WP_246917183.1	restriction endonuclease	-
LCD48_RS02765 (LCD48_02760)	575932..576801	-	870	WP_045889415.1	HNH endonuclease	-
LCD48_RS02770 (LCD48_02765)	577104..577581	+	478	Protein_535	SymE family type I addiction module toxin	-
LCD48_RS02775 (LCD48_02770)	577661..578032	-	372	WP_087823583.1	hypothetical protein	-
LCD48_RS02780 (LCD48_02775)	578148..578627	-	480	WP_039260936.1	type VI secretion system tube protein Hcp	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	546172..580853	34681
	-	flank	IS/Tn	-	-	568567..569547	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-105)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12031.62 Da
• Isoelectric Point: 5.5235

>T217493 WP_192082478.1 NZ_CP083834:573404-573724 [Enterobacter asburiae]
MQISTVPATVPVSSRQSPIQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TRQEQTPFLTATDILRARRATGLINT

Nucleotide

• Length: 321 bp

>T217493 NZ_CP114324:368555-368902 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGAGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11945.60 Da
• Isoelectric Point: 6.5925

>AT217493 WP_246917177.1 NZ_CP083834:573049-573372 [Enterobacter asburiae]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGAFNEEEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLHHN
GLTCEADTLGSHGYVYIAIYPHTPASQ

Nucleotide

• Length: 324 bp

>AT217493 NZ_CP114324:368902-369201 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA