Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 271245..271841 Replicon chromosome
Accession NZ_CP083834
Organism Enterobacter asburiae strain AR0468-yvys

Toxin (Protein)


Gene name PumA Uniprot ID A0A4R0G5K0
Locus tag LCD48_RS01235 Protein ID WP_039264167.1
Coordinates 271539..271841 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A808IFF3
Locus tag LCD48_RS01230 Protein ID WP_033144375.1
Coordinates 271245..271532 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD48_RS01225 (LCD48_01225) 269617..271248 + 1632 WP_039264168.1 Na/Pi cotransporter family protein -
LCD48_RS01230 (LCD48_01230) 271245..271532 - 288 WP_033144375.1 putative addiction module antidote protein Antitoxin
LCD48_RS01235 (LCD48_01235) 271539..271841 - 303 WP_039264167.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCD48_RS01240 (LCD48_01240) 272039..272911 + 873 WP_039264166.1 23S rRNA pseudouridine(2604) synthase RluF -
LCD48_RS01245 (LCD48_01245) 272912..273184 - 273 WP_023309994.1 DUF3811 domain-containing protein -
LCD48_RS01250 (LCD48_01250) 273235..274179 - 945 WP_039264165.1 ketopantoate/pantoate/pantothenate transporter PanS -
LCD48_RS01255 (LCD48_01255) 274273..275622 - 1350 WP_039264164.1 lysine-sensitive aspartokinase 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-93)

Antitoxin

Uncharacterized protein HI1420

(3-89)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11401.17 Da        Isoelectric Point: 9.6038

>T217491 WP_039264167.1 NZ_CP083834:c271841-271539 [Enterobacter asburiae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T217491 NZ_CP114324:301363-301638 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10111.59 Da        Isoelectric Point: 6.4596

>AT217491 WP_033144375.1 NZ_CP083834:c271532-271245 [Enterobacter asburiae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Download         Length: 288 bp

>AT217491 NZ_CP114324:301635-301994 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G5K0

Antitoxin

Source ID Structure
AlphaFold DB A0A808IFF3

References