Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 45948..46585 Replicon plasmid pAR2284_2
Accession NZ_CP083832
Organism Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)


Gene name vagD Uniprot ID A0A9E6WXZ4
Locus tag LCD44_RS25845 Protein ID WP_032662169.1
Coordinates 45948..46358 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A9E6WTF5
Locus tag LCD44_RS25850 Protein ID WP_032662167.1
Coordinates 46355..46585 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD44_RS25825 (LCD44_25870) 41378..42358 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
LCD44_RS25830 (LCD44_25875) 42501..42761 - 261 Protein_43 HNH endonuclease -
LCD44_RS25835 (LCD44_25880) 43305..44327 - 1023 WP_032662174.1 helicase UvrD -
LCD44_RS25840 (LCD44_25885) 44312..45874 - 1563 WP_032662171.1 AAA family ATPase -
LCD44_RS25845 (LCD44_25890) 45948..46358 - 411 WP_032662169.1 PIN domain-containing protein Toxin
LCD44_RS25850 (LCD44_25895) 46355..46585 - 231 WP_032662167.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LCD44_RS25855 (LCD44_25900) 47127..47417 + 291 WP_013087126.1 hypothetical protein -
LCD44_RS25860 (LCD44_25905) 47548..48078 + 531 WP_241510746.1 hypothetical protein -
LCD44_RS25865 (LCD44_25910) 48097..48873 + 777 WP_015572054.1 site-specific integrase -
LCD44_RS25870 (LCD44_25915) 49075..49332 + 258 Protein_51 site-specific integrase -
LCD44_RS25875 (LCD44_25920) 49576..50586 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - galE 1..119051 119051
- flank IS/Tn - - 41378..42358 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14729.03 Da        Isoelectric Point: 5.7351

>T217490 WP_032662169.1 NZ_CP083832:c46358-45948 [Enterobacter asburiae]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 411 bp

>T217490 NZ_CP114322:c14256-13906 [Klebsiella variicola]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8645.77 Da        Isoelectric Point: 4.4692

>AT217490 WP_032662167.1 NZ_CP083832:c46585-46355 [Enterobacter asburiae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Download         Length: 231 bp

>AT217490 NZ_CP114322:c13901-13599 [Klebsiella variicola]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References