Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93050..94673 Replicon plasmid pAR2284_1
Accession NZ_CP083831
Organism Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LCD44_RS24090 Protein ID WP_000088044.1
Coordinates 93348..94673 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LCD44_RS24085 Protein ID WP_012477377.1
Coordinates 93050..93343 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD44_RS24065 (LCD44_24105) 88849..89241 + 393 WP_000341066.1 hypothetical protein -
LCD44_RS24070 (LCD44_24110) 89900..90556 + 657 WP_000462754.1 trypsin-like serine protease -
LCD44_RS24075 (LCD44_24115) 90759..91256 + 498 WP_000340139.1 membrane protein -
LCD44_RS24080 (LCD44_24120) 91261..92649 + 1389 WP_012695445.1 hypothetical protein -
LCD44_RS24085 (LCD44_24125) 93050..93343 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LCD44_RS24090 (LCD44_24130) 93348..94673 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LCD44_RS24095 (LCD44_24135) 94734..94940 + 207 WP_000134171.1 hypothetical protein -
LCD44_RS24100 (LCD44_24140) 95041..95451 + 411 WP_000985909.1 hypothetical protein -
LCD44_RS24105 (LCD44_24145) 95464..95988 + 525 WP_001805097.1 hypothetical protein -
LCD44_RS24110 (LCD44_24150) 96170..97174 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / aac(6')-Ib-cr / blaSFO-1 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / blaTEM-1B / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrA1 / catA1 / dfrA16 / aadA2 / blaCTX-M-9 / mcr-9 - 1..373545 373545


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T217488 WP_000088044.1 NZ_CP083831:93348-94673 [Enterobacter asburiae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T217488 NZ_CP114321:7596-8012 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT217488 WP_012477377.1 NZ_CP083831:93050-93343 [Enterobacter asburiae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT217488 NZ_CP114321:7369-7599 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References