Detailed information of TA system TA217487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4798593..4799209
• Replicon: chromosome
• Accession: NZ_CP083830
• Organism: Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCD44_RS23255
• Protein ID: WP_080298642.1
• Coordinates: 4798593..4798964 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: LCD44_RS23260
• Protein ID: WP_023309753.1
• Coordinates: 4798967..4799209 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD44_RS23240 (LCD44_23280)	4796093..4796995	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
LCD44_RS23245 (LCD44_23285)	4796992..4797627	+	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
LCD44_RS23250 (LCD44_23290)	4797624..4798553	+	930	WP_045404233.1	formate dehydrogenase accessory protein FdhE	-
LCD44_RS23255 (LCD44_23295)	4798593..4798964	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCD44_RS23260 (LCD44_23300)	4798967..4799209	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
LCD44_RS23265 (LCD44_23305)	4799409..4800329	+	921	WP_045404232.1	alpha/beta hydrolase	-
LCD44_RS23270 (LCD44_23310)	4800338..4801279	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
LCD44_RS23275 (LCD44_23315)	4801324..4801761	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
LCD44_RS23280 (LCD44_23320)	4801758..4802639	-	882	WP_023309756.1	virulence factor BrkB family protein	-
LCD44_RS23285 (LCD44_23325)	4802633..4803232	-	600	WP_045404231.1	glucose-1-phosphatase	-
LCD44_RS23290 (LCD44_23330)	4803353..4804153	-	801	WP_045404230.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T217487 WP_080298642.1 NZ_CP083830:c4798964-4798593 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T217487 NZ_CP114320:c5491185-5490802 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT217487 WP_023309753.1 NZ_CP083830:c4799209-4798967 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT217487 NZ_CP114320:c5491427-5491185 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0