Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3754116..3754736 Replicon chromosome
Accession NZ_CP083830
Organism Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag LCD44_RS18235 Protein ID WP_008499287.1
Coordinates 3754518..3754736 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LCD44_RS18230 Protein ID WP_008499288.1
Coordinates 3754116..3754490 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD44_RS18220 (LCD44_18250) 3749242..3750435 + 1194 WP_023310601.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCD44_RS18225 (LCD44_18255) 3750458..3753604 + 3147 WP_023310600.1 multidrug efflux RND transporter permease subunit AcrB -
LCD44_RS18230 (LCD44_18260) 3754116..3754490 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LCD44_RS18235 (LCD44_18265) 3754518..3754736 + 219 WP_008499287.1 HHA domain-containing protein Toxin
LCD44_RS18240 (LCD44_18270) 3754942..3755493 + 552 WP_023310599.1 maltose O-acetyltransferase -
LCD44_RS18245 (LCD44_18275) 3755611..3756078 + 468 WP_045400719.1 YlaC family protein -
LCD44_RS18250 (LCD44_18280) 3756050..3757510 - 1461 WP_045400715.1 PLP-dependent aminotransferase family protein -
LCD44_RS18255 (LCD44_18285) 3757612..3758322 + 711 WP_023310596.1 GNAT family protein -
LCD44_RS18260 (LCD44_18290) 3758319..3758459 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD44_RS18265 (LCD44_18295) 3758462..3758722 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T217485 WP_008499287.1 NZ_CP083830:3754518-3754736 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T217485 NZ_CP114320:4295811-4296029 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT217485 WP_008499288.1 NZ_CP083830:3754116-3754490 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT217485 NZ_CP114320:4295410-4295784 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References