Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 1377735..1378399 Replicon chromosome
Accession NZ_CP083830
Organism Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)


Gene name ykfI Uniprot ID A0A157QTP3
Locus tag LCD44_RS06690 Protein ID WP_007869731.1
Coordinates 1377735..1378061 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0M7FI99
Locus tag LCD44_RS06695 Protein ID WP_007869729.1
Coordinates 1378082..1378399 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD44_RS06650 (LCD44_06660) 1372981..1373118 + 138 WP_162184392.1 hypothetical protein -
LCD44_RS06655 (LCD44_06665) 1373752..1374084 + 333 Protein_1291 type VI secretion system contractile sheath small subunit -
LCD44_RS06660 (LCD44_06670) 1374053..1374346 - 294 WP_241510723.1 OmpA family protein -
LCD44_RS06665 (LCD44_06675) 1374710..1374940 + 231 Protein_1293 ATP-binding protein -
LCD44_RS06670 (LCD44_06680) 1374998..1375126 + 129 WP_229218363.1 hypothetical protein -
LCD44_RS06675 (LCD44_06685) 1375119..1375571 + 453 WP_045402629.1 type VI secretion system baseplate subunit TssE -
LCD44_RS06680 (LCD44_06690) 1375586..1376056 + 471 WP_045402632.1 hypothetical protein -
LCD44_RS06685 (LCD44_06695) 1376160..1377491 + 1332 WP_045402634.1 VasL domain-containing protein -
LCD44_RS06690 (LCD44_06700) 1377735..1378061 - 327 WP_007869731.1 TA system toxin CbtA family protein Toxin
LCD44_RS06695 (LCD44_06705) 1378082..1378399 - 318 WP_007869729.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LCD44_RS06700 (LCD44_06710) 1378413..1378640 - 228 WP_013098172.1 DUF987 domain-containing protein -
LCD44_RS06705 (LCD44_06715) 1378649..1379125 - 477 WP_013098171.1 RadC family protein -
LCD44_RS06710 (LCD44_06720) 1379141..1379599 - 459 WP_013098170.1 antirestriction protein -
LCD44_RS06715 (LCD44_06725) 1379703..1379942 - 240 WP_007869725.1 DUF905 domain-containing protein -
LCD44_RS06720 (LCD44_06730) 1379966..1380787 - 822 WP_007869723.1 DUF932 domain-containing protein -
LCD44_RS06725 (LCD44_06735) 1380914..1381210 - 297 WP_015386464.1 hypothetical protein -
LCD44_RS06730 (LCD44_06740) 1381254..1381526 - 273 WP_007869719.1 hypothetical protein -
LCD44_RS06735 (LCD44_06745) 1381583..1382320 - 738 WP_013098169.1 DeoR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1375586..1410145 34559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-106)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12299.25 Da        Isoelectric Point: 8.9951

>T217475 WP_007869731.1 NZ_CP083830:c1378061-1377735 [Enterobacter asburiae]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Download         Length: 327 bp

>T217475 NZ_CP114318:c14256-13906 [Klebsiella variicola]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11917.60 Da        Isoelectric Point: 7.0264

>AT217475 WP_007869729.1 NZ_CP083830:c1378399-1378082 [Enterobacter asburiae]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT217475 NZ_CP114318:c13901-13599 [Klebsiella variicola]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A157QTP3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M7FI99

References