Detailed information of TA system TA217473

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 863182..863839
• Replicon: chromosome
• Accession: NZ_CP083830
• Organism: Enterobacter asburiae strain AR2284-yvys

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: LCD44_RS04220
• Protein ID: WP_045405042.1
• Coordinates: 863429..863839 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V3PWU7
• Locus tag: LCD44_RS04215
• Protein ID: WP_010435322.1
• Coordinates: 863182..863448 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD44_RS04190 (LCD44_04200)	858364..859797	-	1434	WP_045405037.1	6-phospho-beta-glucosidase BglA	-
LCD44_RS04195 (LCD44_04205)	859914..860645	-	732	WP_032660101.1	MurR/RpiR family transcriptional regulator	-
LCD44_RS04200 (LCD44_04210)	860697..861008	+	312	WP_023309102.1	N(4)-acetylcytidine aminohydrolase	-
LCD44_RS04205 (LCD44_04215)	861174..861833	+	660	WP_023309101.1	hemolysin III family protein	-
LCD44_RS04210 (LCD44_04220)	861907..862887	-	981	WP_045405040.1	tRNA-modifying protein YgfZ	-
LCD44_RS04215 (LCD44_04225)	863182..863448	+	267	WP_010435322.1	FAD assembly factor SdhE	Antitoxin
LCD44_RS04220 (LCD44_04230)	863429..863839	+	411	WP_045405042.1	protein YgfX	Toxin
LCD44_RS04225 (LCD44_04235)	863846..864367	-	522	WP_045405044.1	flavodoxin FldB	-
LCD44_RS04230 (LCD44_04240)	864469..865365	+	897	WP_023309098.1	site-specific tyrosine recombinase XerD	-
LCD44_RS04235 (LCD44_04245)	865394..866107	+	714	WP_045405046.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LCD44_RS04240 (LCD44_04250)	866113..867846	+	1734	WP_045355605.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16157.12 Da
• Isoelectric Point: 10.9865

>T217473 WP_045405042.1 NZ_CP083830:863429-863839 [Enterobacter asburiae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNYGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T217473 NZ_CP114317:7596-8012 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.02 Da
• Isoelectric Point: 5.1340

>AT217473 WP_010435322.1 NZ_CP083830:863182-863448 [Enterobacter asburiae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT217473 NZ_CP114317:7369-7599 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: V3PWU7