Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1085210..1085830 Replicon chromosome
Accession NZ_CP083828
Organism Enterobacter kobei strain 11778-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag LCD42_RS05065 Protein ID WP_014882802.1
Coordinates 1085210..1085428 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LCD42_RS05070 Protein ID WP_008499288.1
Coordinates 1085456..1085830 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD42_RS05035 (LCD42_05045) 1081219..1081479 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
LCD42_RS05040 (LCD42_05050) 1081482..1081622 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD42_RS05045 (LCD42_05055) 1081619..1082329 - 711 WP_014882798.1 GNAT family protein -
LCD42_RS05050 (LCD42_05060) 1082431..1083891 + 1461 WP_058690566.1 PLP-dependent aminotransferase family protein -
LCD42_RS05055 (LCD42_05065) 1083863..1084330 - 468 WP_014882800.1 YlaC family protein -
LCD42_RS05060 (LCD42_05070) 1084449..1085000 - 552 WP_014882801.1 maltose O-acetyltransferase -
LCD42_RS05065 (LCD42_05075) 1085210..1085428 - 219 WP_014882802.1 HHA domain-containing protein Toxin
LCD42_RS05070 (LCD42_05080) 1085456..1085830 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LCD42_RS05075 (LCD42_05085) 1086342..1089488 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
LCD42_RS05080 (LCD42_05090) 1089511..1090704 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T217461 WP_014882802.1 NZ_CP083828:c1085428-1085210 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T217461 NZ_CP114316:301363-301638 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT217461 WP_008499288.1 NZ_CP083828:c1085830-1085456 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT217461 NZ_CP114316:301635-301994 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References