Detailed information of TA system TA217459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3892531..3893312
• Replicon: chromosome
• Accession: NZ_CP083821
• Organism: Enterobacter cloacae subsp. cloacae strain 12961-yvys

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LCD47_RS18610
• Protein ID: WP_139937116.1
• Coordinates: 3892531..3893022 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A5E1AUV7
• Locus tag: LCD47_RS18615
• Protein ID: WP_029882966.1
• Coordinates: 3893019..3893312 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD47_RS18590 (LCD47_18620)	3888271..3888963	+	693	WP_013098687.1	response regulator	-
LCD47_RS18595 (LCD47_18625)	3889062..3891197	-	2136	WP_230794071.1	ornithine decarboxylase	-
LCD47_RS18600 (LCD47_18630)	3891380..3892093	+	714	WP_139937117.1	DUF554 domain-containing protein	-
LCD47_RS18610 (LCD47_18640)	3892531..3893022	-	492	WP_139937116.1	GNAT family N-acetyltransferase	Toxin
LCD47_RS18615 (LCD47_18645)	3893019..3893312	-	294	WP_029882966.1	DUF1778 domain-containing protein	Antitoxin
LCD47_RS18620 (LCD47_18650)	3893423..3894235	-	813	WP_128569456.1	DUF817 domain-containing protein	-
LCD47_RS18625 (LCD47_18655)	3894329..3896191	-	1863	WP_139937115.1	bifunctional glutathionylspermidine amidase/synthase	-
LCD47_RS18630 (LCD47_18660)	3896375..3897244	+	870	WP_014833255.1	glutathione-dependent disulfide-bond oxidoreductase	-
LCD47_RS18635 (LCD47_18665)	3897405..3897737	+	333	WP_139937114.1	glycine zipper domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17644.50 Da
• Isoelectric Point: 7.2653

>T217459 WP_139937116.1 NZ_CP083821:c3893022-3892531 [Enterobacter cloacae subsp. cloacae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVSGASRTFVCCGNDSNVQAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLM
ASI

Nucleotide

• Length: 492 bp

>T217459 NZ_CP114313:67656-68138 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11003.66 Da
• Isoelectric Point: 9.9156

>AT217459 WP_029882966.1 NZ_CP083821:c3893312-3893019 [Enterobacter cloacae subsp. cloacae]
MPAANTMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQAPAPN
ATLRKTMQTPAPWEREK

Nucleotide

• Length: 294 bp

>AT217459 NZ_CP114313:67402-67668 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AUV7