Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | ykfI-yfjZ/CbtA-CbeA |
| Location | 3285851..3286515 | Replicon | chromosome |
| Accession | NZ_CP083821 | ||
| Organism | Enterobacter cloacae subsp. cloacae strain 12961-yvys | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | A0A157QTP3 |
| Locus tag | LCD47_RS15660 | Protein ID | WP_007869731.1 |
| Coordinates | 3286189..3286515 (+) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | yfjZ | Uniprot ID | A0A0M7FI99 |
| Locus tag | LCD47_RS15655 | Protein ID | WP_007869729.1 |
| Coordinates | 3285851..3286168 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCD47_RS15610 (LCD47_15635) | 3280938..3281669 | + | 732 | WP_230794038.1 | 50S ribosome-binding GTPase | - |
| LCD47_RS15615 (LCD47_15640) | 3281930..3282667 | + | 738 | WP_230794039.1 | DeoR family transcriptional regulator | - |
| LCD47_RS15620 (LCD47_15645) | 3282724..3282996 | + | 273 | WP_230794040.1 | hypothetical protein | - |
| LCD47_RS15625 (LCD47_15650) | 3283040..3283336 | + | 297 | WP_015386464.1 | hypothetical protein | - |
| LCD47_RS15630 (LCD47_15655) | 3283463..3284284 | + | 822 | WP_007869723.1 | DUF932 domain-containing protein | - |
| LCD47_RS15635 (LCD47_15660) | 3284308..3284547 | + | 240 | WP_007869725.1 | DUF905 domain-containing protein | - |
| LCD47_RS15640 (LCD47_15665) | 3284651..3285109 | + | 459 | WP_013098170.1 | antirestriction protein | - |
| LCD47_RS15645 (LCD47_15670) | 3285125..3285601 | + | 477 | WP_013098171.1 | RadC family protein | - |
| LCD47_RS15650 (LCD47_15675) | 3285610..3285837 | + | 228 | WP_013098172.1 | DUF987 domain-containing protein | - |
| LCD47_RS15655 (LCD47_15680) | 3285851..3286168 | + | 318 | WP_007869729.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| LCD47_RS15660 (LCD47_15685) | 3286189..3286515 | + | 327 | WP_007869731.1 | TA system toxin CbtA family protein | Toxin |
| LCD47_RS15665 (LCD47_15690) | 3286725..3288104 | - | 1380 | WP_040022349.1 | diguanylate cyclase | - |
| LCD47_RS15670 (LCD47_15695) | 3288416..3289336 | + | 921 | WP_167816581.1 | kdo(2)-lipid IV(A) palmitoleoyltransferase | - |
| LCD47_RS15675 (LCD47_15700) | 3289694..3289765 | + | 72 | WP_099458937.1 | membrane protein YpdK | - |
| LCD47_RS15680 (LCD47_15705) | 3289821..3291056 | - | 1236 | WP_020690608.1 | alanine transaminase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 12299.25 Da Isoelectric Point: 8.9951
>T217457 WP_007869731.1 NZ_CP083821:3286189-3286515 [Enterobacter cloacae subsp. cloacae]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH
Download Length: 327 bp
>T217457 NZ_CP114312:c5490119-5489736 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11917.60 Da Isoelectric Point: 7.0264
>AT217457 WP_007869729.1 NZ_CP083821:3285851-3286168 [Enterobacter cloacae subsp. cloacae]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT217457 NZ_CP114312:c5490361-5490119 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A157QTP3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M7FI99 |