Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1093310..1093930 Replicon chromosome
Accession NZ_CP083821
Organism Enterobacter cloacae subsp. cloacae strain 12961-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag LCD47_RS05200 Protein ID WP_013095889.1
Coordinates 1093310..1093528 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LCD47_RS05205 Protein ID WP_008499288.1
Coordinates 1093556..1093930 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD47_RS05170 (LCD47_05180) 1089322..1089582 + 261 WP_139937107.1 type B 50S ribosomal protein L31 -
LCD47_RS05175 (LCD47_05185) 1089585..1089725 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD47_RS05180 (LCD47_05190) 1089722..1090432 - 711 WP_023620096.1 GNAT family protein -
LCD47_RS05185 (LCD47_05195) 1090534..1091994 + 1461 WP_230793807.1 PLP-dependent aminotransferase family protein -
LCD47_RS05190 (LCD47_05200) 1091966..1092433 - 468 WP_013095887.1 YlaC family protein -
LCD47_RS05195 (LCD47_05205) 1092549..1093100 - 552 WP_013095888.1 maltose O-acetyltransferase -
LCD47_RS05200 (LCD47_05210) 1093310..1093528 - 219 WP_013095889.1 HHA domain-containing protein Toxin
LCD47_RS05205 (LCD47_05215) 1093556..1093930 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LCD47_RS05210 (LCD47_05220) 1094443..1097589 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
LCD47_RS05215 (LCD47_05225) 1097612..1098805 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T217445 WP_013095889.1 NZ_CP083821:c1093528-1093310 [Enterobacter cloacae subsp. cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T217445 NZ_CP114309:58852-59334 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT217445 WP_008499288.1 NZ_CP083821:c1093930-1093556 [Enterobacter cloacae subsp. cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT217445 NZ_CP114309:58598-58864 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References