Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 631848..632642 Replicon chromosome
Accession NZ_CP083821
Organism Enterobacter cloacae subsp. cloacae strain 12961-yvys

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag LCD47_RS03025 Protein ID WP_013095427.1
Coordinates 631848..632369 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag LCD47_RS03030 Protein ID WP_013095428.1
Coordinates 632373..632642 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD47_RS03000 (LCD47_03005) 627175..627336 + 162 WP_139937123.1 DUF1127 domain-containing protein -
LCD47_RS03005 (LCD47_03010) 627423..628811 - 1389 WP_230793776.1 PLP-dependent aminotransferase family protein -
LCD47_RS03010 (LCD47_03015) 628914..629393 + 480 WP_013095424.1 carboxymuconolactone decarboxylase family protein -
LCD47_RS03015 (LCD47_03020) 629380..630282 - 903 WP_139937125.1 LysR family transcriptional regulator -
LCD47_RS03020 (LCD47_03025) 630383..631798 + 1416 WP_230793777.1 aldehyde dehydrogenase family protein -
LCD47_RS03025 (LCD47_03030) 631848..632369 - 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
LCD47_RS03030 (LCD47_03035) 632373..632642 - 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
LCD47_RS03035 (LCD47_03040) 632857..633180 + 324 WP_023620659.1 antibiotic biosynthesis monooxygenase -
LCD47_RS03040 (LCD47_03045) 633173..633571 + 399 WP_029882197.1 hypothetical protein -
LCD47_RS03045 (LCD47_03050) 633543..634238 + 696 WP_038418621.1 winged helix-turn-helix domain-containing protein -
LCD47_RS03050 (LCD47_03055) 634442..634912 + 471 WP_013095432.1 MarR family transcriptional regulator -
LCD47_RS03055 (LCD47_03060) 634909..635976 + 1068 WP_029882194.1 HlyD family secretion protein -
LCD47_RS03060 (LCD47_03065) 635966..637042 + 1077 WP_013095434.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T217444 WP_013095427.1 NZ_CP083821:c632369-631848 [Enterobacter cloacae subsp. cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T217444 NZ_CP114308:c4697201-4696944 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT217444 WP_013095428.1 NZ_CP083821:c632642-632373 [Enterobacter cloacae subsp. cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT217444 NZ_CP114308:c4696933-4696382 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGACCTGGTTGCCGATTTGCTCAACGATGAAAGTGCTCACCCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCAGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCACTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References