Detailed information of TA system TA217441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4918..5654
• Replicon: plasmid p13840_1
• Accession: NZ_CP083820
• Organism: Enterobacter roggenkampii strain 13840-yvys

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LCD41_RS22920
• Protein ID: WP_003026803.1
• Coordinates: 5172..5654 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LCD41_RS22915
• Protein ID: WP_003026799.1
• Coordinates: 4918..5184 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD41_RS22890 (LCD41_22940)	1..1011	+	1011	WP_000200070.1	RepB family plasmid replication initiator protein	-
LCD41_RS22895 (LCD41_22945)	1709..2449	-	741	WP_001515717.1	tyrosine-type recombinase/integrase	-
LCD41_RS22900 (LCD41_22950)	2813..3781	-	969	WP_023307223.1	IS5-like element IS903B family transposase	-
LCD41_RS22905 (LCD41_22955)	4026..4229	+	204	WP_004213596.1	HHA domain-containing protein	-
LCD41_RS22910 (LCD41_22960)	4243..4473	+	231	WP_023307222.1	hypothetical protein	-
LCD41_RS22915 (LCD41_22965)	4918..5184	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LCD41_RS22920 (LCD41_22970)	5172..5654	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LCD41_RS22925 (LCD41_22975)	5866..7209	+	1344	WP_080339306.1	ISNCY family transposase	-
LCD41_RS22930 (LCD41_22980)	7280..8098	-	819	WP_023307220.1	abortive infection family protein	-
LCD41_RS22935 (LCD41_22985)	8107..8667	-	561	WP_247170004.1	recombinase family protein	-
LCD41_RS22940 (LCD41_22990)	8692..9396	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LCD41_RS22945 (LCD41_22995)	9451..10643	+	1193	Protein_11	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-10	mrkF / mrkD / mrkC / mrkB / mrkA	1..94721	94721
	-	inside	IScluster/Tn	mcr-10	-	2813..28350	25537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T217441 WP_003026803.1 NZ_CP083820:5172-5654 [Enterobacter roggenkampii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T217441 NZ_CP114308:926426-926836 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT217441 WP_003026799.1 NZ_CP083820:4918-5184 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT217441 NZ_CP114308:926179-926445 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66