Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 528885..529461 Replicon chromosome
Accession NZ_CP083819
Organism Enterobacter roggenkampii strain 13840-yvys

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag LCD41_RS02545 Protein ID WP_025912099.1
Coordinates 528885..529172 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag LCD41_RS02550 Protein ID WP_023293359.1
Coordinates 529159..529461 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD41_RS02515 (LCD41_02525) 523897..524013 + 117 Protein_484 amino acid-binding protein -
LCD41_RS02520 (LCD41_02530) 524006..524701 + 696 WP_047746532.1 winged helix-turn-helix domain-containing protein -
LCD41_RS02525 (LCD41_02535) 524847..525317 + 471 WP_047746530.1 MarR family transcriptional regulator -
LCD41_RS02530 (LCD41_02540) 525314..526381 + 1068 WP_025912094.1 HlyD family secretion protein -
LCD41_RS02535 (LCD41_02545) 526371..527447 + 1077 WP_047746527.1 DUF2955 domain-containing protein -
LCD41_RS02540 (LCD41_02550) 527444..528715 - 1272 WP_025912097.1 DUF445 domain-containing protein -
LCD41_RS02545 (LCD41_02555) 528885..529172 + 288 WP_025912099.1 BrnT family toxin Toxin
LCD41_RS02550 (LCD41_02560) 529159..529461 + 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
LCD41_RS02555 (LCD41_02565) 529492..530130 - 639 WP_047746523.1 LysE family translocator -
LCD41_RS02560 (LCD41_02570) 530178..530921 - 744 WP_025912104.1 AraC family transcriptional regulator -
LCD41_RS02565 (LCD41_02575) 531073..532443 + 1371 WP_047746521.1 NAD-dependent succinate-semialdehyde dehydrogenase -
LCD41_RS02570 (LCD41_02580) 532489..532809 - 321 WP_008501314.1 hypothetical protein -
LCD41_RS02575 (LCD41_02585) 532809..533354 - 546 WP_047746519.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T217427 WP_025912099.1 NZ_CP083819:528885-529172 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T217427 NZ_CP114281:c1813187-1812804 [Gallibacterium anatis]
ATGAGAATATTTAAAACTAAAGCTTTTAACAAATTTGCTTTAAAAAATGGTATTCCTGACAGCGAATTAATCAATGCTAT
AACGAGGGCAGAGCAAGGATTAATAGATGCTGATTTAGGCAGTAATATTATTAAGCAGAGAATAGCCAGAAAAGGGCAGG
TACGTAGTGGTGGATTCCGTTCTTTTATTTTTTATTGTATCCAAAATAACAGTTATTTTGTTGCCGGAATTAGTAAAAAT
GACAGAGAAAATATTTCATCACAAGAGTTAGCAGCGTTAAAGGCATTGGCTAGAGAATATACTCGACTAACAGCAAAACA
GATTGAAGCACAGATCGAAAACGGATTATTCATCGAAATTTTACCGGAGGCAGAGGATGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT217427 WP_023293359.1 NZ_CP083819:529159-529461 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT217427 NZ_CP114281:c1812811-1812509 [Gallibacterium anatis]
ATGAGTGAATTGTTGGCAATGATCCACGAAAATGCGGCGGATTTACATAAAGCCGGCATTATTAATAAAAAAACAATGAA
AGAATTTGATCAGCTTTGTTTGACACCTGTTGTAACTTATACGCCGGACGAAATTAGAGCGATTCGTGAAAAAGAACAGG
TATCCCAAGCGGTATTCGCACATTATCTTAATGTGAGTAAAAATATGATTTCCGAATGGGAACGAGGCGTTAAAAAACCA
AGTGGTGCAGCATTAAAGTTGCTTAATTTGGTACAGCATAATGGTATTGAGATTCTTGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References