Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 564901..565477 Replicon chromosome
Accession NZ_CP083815
Organism Enterobacter asburiae strain 161373-yvys

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag LCD39_RS02695 Protein ID WP_010427219.1
Coordinates 564901..565188 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag LCD39_RS02700 Protein ID WP_033144548.1
Coordinates 565175..565477 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD39_RS02670 (LCD39_02670) 560017..560712 + 696 WP_232938659.1 winged helix-turn-helix domain-containing protein -
LCD39_RS02675 (LCD39_02675) 560862..561332 + 471 WP_246946964.1 MarR family transcriptional regulator -
LCD39_RS02680 (LCD39_02680) 561329..562396 + 1068 WP_139153136.1 HlyD family secretion protein -
LCD39_RS02685 (LCD39_02685) 562386..563462 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
LCD39_RS02690 (LCD39_02690) 563459..564730 - 1272 WP_246946965.1 DUF445 domain-containing protein -
LCD39_RS02695 (LCD39_02695) 564901..565188 + 288 WP_010427219.1 BrnT family toxin Toxin
LCD39_RS02700 (LCD39_02700) 565175..565477 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
LCD39_RS02705 (LCD39_02705) 565508..566146 - 639 WP_033144549.1 LysE family translocator -
LCD39_RS02710 (LCD39_02710) 566194..566937 - 744 WP_246946966.1 AraC family transcriptional regulator -
LCD39_RS02715 (LCD39_02715) 567089..568459 + 1371 WP_246946967.1 NAD-dependent succinate-semialdehyde dehydrogenase -
LCD39_RS02720 (LCD39_02720) 568504..568824 - 321 WP_039262264.1 hypothetical protein -
LCD39_RS02725 (LCD39_02725) 568824..569369 - 546 WP_023310269.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T217410 WP_010427219.1 NZ_CP083815:564901-565188 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T217410 NZ_CP114280:c1140488-1140084 [Dickeya lacustris]
ATGTTGAAATACCTGCTTGATACCAACATTTGCATTTACACCATAAAGAACAAGCCGCAAGAGGTCAGAGAGGCGTTCCA
GCGTTATTACGGGCAATTTGCGATCAGTTCTATCACGTTGATGGAGCTTATCTATGGCGCGGAAAAGTCGGCTAATCCAG
AAAAGAATCTGGCGGTAATAGAAGGATTTTCCGCACGTCTGGACATCCAGTCCTATGGGTTTGATGCTGCCGTACATACC
GGTCAAATTCGTGCCGAGCTGGCGAAACAAGGAACCCCCATAGGCCCGTATGATGCCATGTTAGCCGGTCACGCGCGATC
CGCAGGTTTAATACTGATAACAAACAATGTACGCGAATTTGAGCGTGTACCTGGGCTACGAGTGGAAAACTGGGTAAGCC
GATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT217410 WP_033144548.1 NZ_CP083815:565175-565477 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT217410 NZ_CP114280:c1140718-1140488 [Dickeya lacustris]
ATGGAAAAAACCACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCTAAAGCTGTTGCGTTACCCGATGATGT
TAGGCATGTCGATATTGTCGCCATTGGCCGCACGCGTATCATTACTCCGGCAGGCGAAAGCTGGGACAATTGGTTTGATG
AAGCGGGCGTGACACCGGATTTCATGAGCACCAGAGAGCAACCGGATGAACAGATCAGGGACGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References