Detailed information of TA system TA217408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 251186..251778
• Replicon: chromosome
• Accession: NZ_CP083815
• Organism: Enterobacter asburiae strain 161373-yvys

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LCD39_RS01135
• Protein ID: WP_033144376.1
• Coordinates: 251476..251778 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A808IFF3
• Locus tag: LCD39_RS01130
• Protein ID: WP_033144375.1
• Coordinates: 251186..251473 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD39_RS01125 (LCD39_01125)	249558..251189	+	1632	WP_033144374.1	Na/Pi cotransporter family protein	-
LCD39_RS01130 (LCD39_01130)	251186..251473	-	288	WP_033144375.1	putative addiction module antidote protein	Antitoxin
LCD39_RS01135 (LCD39_01135)	251476..251778	-	303	WP_033144376.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD39_RS01140 (LCD39_01140)	251976..252848	+	873	WP_023309993.1	23S rRNA pseudouridine(2604) synthase RluF	-
LCD39_RS01145 (LCD39_01145)	252849..253121	-	273	WP_023309994.1	DUF3811 domain-containing protein	-
LCD39_RS01150 (LCD39_01150)	253172..254116	-	945	WP_048977786.1	ketopantoate/pantoate/pantothenate transporter PanS	-
LCD39_RS01155 (LCD39_01155)	254210..255559	-	1350	WP_246946881.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11284.02 Da
• Isoelectric Point: 9.9325

>T217408 WP_033144376.1 NZ_CP083815:c251778-251476 [Enterobacter asburiae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNPNGRSE

Nucleotide

• Length: 303 bp

>T217408 NZ_CP114278:2915669-2915986 [Brevundimonas vesicularis]
GTGAGCGCCAGATATTCCAATGCTGCGAAACGCGATCTGATTGCCATCTATCTTGATGGTGCGGCGAAGTTTGGATCGCG
GCAAGCTGCAGCCTACACAACAGGTCTCGGCGCCACCGTCCGAATGATCAGCGACTACCCTCTTTCGTCTCGCCTCAGGA
CCGAGCTCGATCTGCCGGTCCGCGTGCGAACCTACAAAGCTCACGTCATTATCTACGTGATAGAAGAAGGTGGGGTGTTG
GTGCTTCGGTTTCGTCACGGGCACGAAGATTGGCAGTCAGATCCACTCGGTGGTGACTTGGACAAGGATAAACCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10111.59 Da
• Isoelectric Point: 6.4596

>AT217408 WP_033144375.1 NZ_CP083815:c251473-251186 [Enterobacter asburiae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Nucleotide

• Length: 288 bp

>AT217408 NZ_CP114278:2915404-2915661 [Brevundimonas vesicularis]
ATGGCGACGATGAACATCTCCCTGCCCGATCCGATGAAGGCCTGGGTCGAGGAACAGGCGAAATCCGGGCGCTACGCCAA
CACGTCCGACGTGGTGCGCGATCTGATCCGCCGCGAGCAGGTCAAGGCCGAGAAGATCGCCCACTGGAACACTCTGATCG
AAGAGGCCTACGCCTCCGGGATCAGCGAAATGACGTTCGAGGAGATTATCGGTGAGGCCCGCGCGGAAGCGCAGGCTCGG
GCTTCTCGCGCAGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A808IFF3