Detailed information of TA system TA217406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 78675..79318
• Replicon: plasmid p33.1_2
• Accession: NZ_CP083810
• Organism: Pantoea agglomerans strain 33.1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LB453_RS22555
• Protein ID: WP_103797223.1
• Coordinates: 78902..79318 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LB453_RS22550
• Protein ID: WP_048782087.1
• Coordinates: 78675..78905 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB453_RS22535 (LB453_22470)	75046..76155	+	1110	WP_033755647.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
LB453_RS22540 (LB453_22475)	76197..77498	+	1302	WP_033755645.1	maltoporin	-
LB453_RS22545 (LB453_22480)	77617..78531	+	915	WP_103797222.1	maltose operon protein MalM	-
LB453_RS22550 (LB453_22485)	78675..78905	+	231	WP_048782087.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LB453_RS22555 (LB453_22490)	78902..79318	+	417	WP_103797223.1	type II toxin-antitoxin system VapC family toxin	Toxin
LB453_RS22560 (LB453_22495)	79390..81459	-	2070	WP_103797224.1	4-alpha-glucanotransferase	-
LB453_RS22565 (LB453_22500)	81468..83870	-	2403	WP_224481801.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207704	207704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15468.01 Da
• Isoelectric Point: 7.9336

>T217406 WP_103797223.1 NZ_CP083810:78902-79318 [Pantoea agglomerans]
VIKTYMLDTNICSFIMREQPEAVLMRLEQAVLRRHRIVVSAITYAEMRFGAVGKKASPRHVQLVEAFCARLDAILAWDRH
AVDATTEIKRALTAIDRPIGPNDTAIAGHAIAADAMLVTHNVKEFERVPGLVFEDWSK

Nucleotide

• Length: 417 bp

>T217406 NZ_CP114278:2910205-2910462 [Brevundimonas vesicularis]
ATGAACGCGATGTCCGTCACCGAACTCCGCAAGAACCTCGCCGCGGCCATCGACCGGGTGACGGCGGATCATGACTACAC
GATCATCACGCGCGAGGGCGGTAAGCCGGCGGCGGTGCTGATGTCGTTGGAGGACTTCGCCTCCTGGCAGGAGACGGAGT
ATCTACTGCGCAGCCCGGCGAACGCTGCGAGGTTGCGTGAGGCGATCTCCGAACTCGATGCGGGCGGCGGCCAGGTTCGC
GAACTGATCGAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8817.87 Da
• Isoelectric Point: 4.4992

>AT217406 WP_048782087.1 NZ_CP083810:78675-78905 [Pantoea agglomerans]
MRTVSIFKNGNNRAIRLPRDLDFDGVNELEIIREGDSLILRPVKPSWGSFREQEKADADFMTERADVFSDEGRFEL

Nucleotide

• Length: 231 bp

>AT217406 NZ_CP114278:2910459-2910719 [Brevundimonas vesicularis]
ATGAAGCTGACCTTCCAGACCTACGGGTGGGAGGATTACCTTCACTGGCAAGACCATGATCGAAAGATGCTGCGAAAGCT
CAATCGGCTGATCATGGAATGCCAGCGGACGCCTTTTTCCGGGACTGGAAAACCTGAACCGTTGAAGGGCGATTTTTCGG
GATGGTGGTCGCGGCGCATCGACCAGGAACATCGGCTCGTCTATCGCGTGACCGACGATGCGCTTTTGATCGCCCAATGC
CGCTATCACTACGACCGATGA