Detailed information of TA system TA217403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3001702..3002321
• Replicon: chromosome
• Accession: NZ_CP083809
• Organism: Pantoea agglomerans strain 33.1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2J9E6F3
• Locus tag: LB453_RS17160
• Protein ID: WP_033752683.1
• Coordinates: 3002103..3002321 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LB453_RS17155
• Protein ID: WP_224481530.1
• Coordinates: 3001702..3002079 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB453_RS17125 (LB453_17075)	2998022..2998273	+	252	WP_103795076.1	type B 50S ribosomal protein L31	-
LB453_RS17130 (LB453_17080)	2998285..2998425	+	141	WP_103795077.1	type B 50S ribosomal protein L36	-
LB453_RS17135 (LB453_17085)	2998467..2999345	-	879	WP_103795078.1	metal ABC transporter substrate-binding protein	-
LB453_RS17140 (LB453_17090)	2999358..3000197	-	840	WP_103795079.1	metal ABC transporter permease	-
LB453_RS17145 (LB453_17095)	3000194..3000850	-	657	WP_103795080.1	ABC transporter ATP-binding protein	-
LB453_RS17150 (LB453_17100)	3001200..3001553	+	354	WP_103795081.1	hypothetical protein	-
LB453_RS17155 (LB453_17105)	3001702..3002079	+	378	WP_224481530.1	Hha toxicity modulator TomB	Antitoxin
LB453_RS17160 (LB453_17110)	3002103..3002321	+	219	WP_033752683.1	HHA domain-containing protein	Toxin
LB453_RS17170 (LB453_17120)	3002711..3003022	+	312	WP_103795083.1	MGMT family protein	-
LB453_RS17175 (LB453_17125)	3003055..3003612	-	558	WP_224481531.1	YbaY family lipoprotein	-
LB453_RS17180 (LB453_17130)	3003812..3004675	+	864	WP_033788820.1	acyl-CoA thioesterase II	-
LB453_RS17185 (LB453_17135)	3004769..3006055	-	1287	WP_033788819.1	ammonium transporter AmtB	-
LB453_RS17190 (LB453_17140)	3006089..3006427	-	339	WP_033752690.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8614.92 Da
• Isoelectric Point: 8.9007

>T217403 WP_033752683.1 NZ_CP083809:3002103-3002321 [Pantoea agglomerans]
MNDKNLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Nucleotide

• Length: 219 bp

>T217403 NZ_CP114266:12377-12640 [Acinetobacter sp. TR3]
ATGACGAATCGTAACGTCGAATGGACTGATAATGCCTGGGATGAATATATCTATTGGCAGACACAGGATAAAAAGATACT
TAAGCGTATTAATACCTTAATCAAAGAATGTCAGCGAACACCTTTTGAAGGAACAGGAAAACCAGAACCTTTAAAAGCTA
ATCTTTCAGGATTTTGGAGTCGTAGGATTGATGAAAAGCATAGATTAGTTTATGAAGTGACAGATGAACGAATCTCTATA
ATTCAATGTCGATTCCATTACTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14733.29 Da
• Isoelectric Point: 4.2410

>AT217403 WP_224481530.1 NZ_CP083809:3001702-3002079 [Pantoea agglomerans]
MDEYSPKRHDIAQLKFLCENLFDESMATLTDSHHGWLNDPTSENNLQLNDLIEHIASFTMNYKIKHAEDEDLITQIDDYL
DDTFMLFTNYGINAQDLNQWQRSARRLFNLFSEECAFLQQPSHSI

Nucleotide

• Length: 378 bp

>AT217403 NZ_CP114266:12127-12390 [Acinetobacter sp. TR3]
ATGCATGTATTTACATATACGGACGCACGTAACAATTTAAAATCTGTTTTAGATAAAGTAATTGATGATGTAGACGTTGC
TTTTATTACAAGAAAAGAAGGTGGCCATGCTGTAGTAATGGGACAAGATCATTATGATAGCTTAATGGAAACGTTATATC
TCCTTTCATCTCCAAATAATGCAAGTCGTTTAAATGAATCAATTGCTCAATTACGTGCAGGGAAAACAAAACACAGAGAG
TTGATTGAGGATGACGAATCGTAA

Structures

Toxin

• AlphaFold DB: A0A2J9E6F3