Detailed information of TA system TA217377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 158279..159189
• Replicon: plasmid pINF019_1
• Accession: NZ_CP083797
• Organism: Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MBW7
• Locus tag: LB481_RS27055
• Protein ID: WP_023329005.1
• Coordinates: 158279..158749 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LB481_RS27060
• Protein ID: WP_023329004.1
• Coordinates: 158746..159189 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB481_RS27030 (LB481_27030)	153970..154938	-	969	WP_072206835.1	IS5 family transposase	-
LB481_RS27035 (LB481_27035)	155226..155474	+	249	WP_004187025.1	plasmid stabilization protein	-
LB481_RS27040 (LB481_27040)	155464..155748	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LB481_RS27045 (LB481_27045)	155764..157107	-	1344	WP_032738442.1	ISNCY family transposase	-
LB481_RS27050 (LB481_27050)	157909..158097	+	189	WP_023329006.1	hypothetical protein	-
LB481_RS27055 (LB481_27055)	158279..158749	-	471	WP_023329005.1	RES family NAD+ phosphorylase	Toxin
LB481_RS27060 (LB481_27060)	158746..159189	-	444	WP_023329004.1	DUF2384 domain-containing protein	Antitoxin
LB481_RS27065 (LB481_27065)	159311..159977	-	667	Protein_186	DUF4113 domain-containing protein	-
LB481_RS27070 (LB481_27070)	160159..161154	-	996	WP_032738441.1	DUF2891 domain-containing protein	-
LB481_RS27075 (LB481_27075)	161164..162150	-	987	Protein_188	DUF979 domain-containing protein	-
LB481_RS27080 (LB481_27080)	162147..162868	-	722	Protein_189	DUF969 domain-containing protein	-
LB481_RS27085 (LB481_27085)	163067..163297	+	231	Protein_190	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..283677	283677
	-	flank	IS/Tn	-	-	153970..154938	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17459.70 Da
• Isoelectric Point: 4.4829

>T217377 WP_023329005.1 NZ_CP083797:c158749-158279 [Klebsiella pneumoniae]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T217377 NZ_CP114186:1439645-1439947 [Vibrio parahaemolyticus]
ATGGCTGAAATAATCTGGACTGAGCCAGCGTTATCAGACCTCAACGATATCGCTGAATACATCGCACTTGAAAATATAGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAAAGTCGAACGCCTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCTTGTTGAGTAAGCAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16477.82 Da
• Isoelectric Point: 10.2498

>AT217377 WP_023329004.1 NZ_CP083797:c159189-158746 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADSVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT217377 NZ_CP114186:1439400-1439657 [Vibrio parahaemolyticus]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCGTATCTAGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAGTGGTAAGTCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBW7