Detailed information of TA system TA217376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 155226..155748
• Replicon: plasmid pINF019_1
• Accession: NZ_CP083797
• Organism: Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: LB481_RS27040
• Protein ID: WP_004187019.1
• Coordinates: 155464..155748 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: LB481_RS27035
• Protein ID: WP_004187025.1
• Coordinates: 155226..155474 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB481_RS27015 (LB481_27015)	151080..151637	-	558	WP_023329012.1	HutD family protein	-
LB481_RS27020 (LB481_27020)	151634..152395	-	762	WP_023329011.1	histidine utilization repressor	-
LB481_RS27025 (LB481_27025)	152498..153865	+	1368	WP_023329010.1	formimidoylglutamate deiminase	-
LB481_RS27030 (LB481_27030)	153970..154938	-	969	WP_072206835.1	IS5 family transposase	-
LB481_RS27035 (LB481_27035)	155226..155474	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
LB481_RS27040 (LB481_27040)	155464..155748	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LB481_RS27045 (LB481_27045)	155764..157107	-	1344	WP_032738442.1	ISNCY family transposase	-
LB481_RS27050 (LB481_27050)	157909..158097	+	189	WP_023329006.1	hypothetical protein	-
LB481_RS27055 (LB481_27055)	158279..158749	-	471	WP_023329005.1	RES family NAD+ phosphorylase	-
LB481_RS27060 (LB481_27060)	158746..159189	-	444	WP_023329004.1	DUF2384 domain-containing protein	-
LB481_RS27065 (LB481_27065)	159311..159977	-	667	Protein_186	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..283677	283677
	-	flank	IS/Tn	-	-	153970..154938	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T217376 WP_004187019.1 NZ_CP083797:155464-155748 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T217376 NZ_CP114180:c1257603-1256857 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT217376 WP_004187025.1 NZ_CP083797:155226-155474 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT217376 NZ_CP114180:c1256856-1256686 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4