Detailed information of TA system TA217375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 138251..138987
• Replicon: plasmid pINF019_1
• Accession: NZ_CP083797
• Organism: Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LB481_RS26950
• Protein ID: WP_023329018.1
• Coordinates: 138251..138733 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LB481_RS26955
• Protein ID: WP_003026799.1
• Coordinates: 138721..138987 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB481_RS26925 (LB481_26925)	133312..134280	+	969	WP_077254073.1	IS5 family transposase	-
LB481_RS26930 (LB481_26930)	134523..134837	+	315	WP_224451391.1	hypothetical protein	-
LB481_RS26935 (LB481_26935)	135231..135803	+	573	WP_032738445.1	recombinase family protein	-
LB481_RS26940 (LB481_26940)	135812..136630	+	819	WP_023307220.1	abortive infection family protein	-
LB481_RS26945 (LB481_26945)	136701..138044	-	1344	WP_077263966.1	ISNCY family transposase	-
LB481_RS26950 (LB481_26950)	138251..138733	-	483	WP_023329018.1	GNAT family N-acetyltransferase	Toxin
LB481_RS26955 (LB481_26955)	138721..138987	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LB481_RS26960 (LB481_26960)	140091..140270	+	180	Protein_166	transposase	-
LB481_RS26970 (LB481_26970)	140523..141332	-	810	WP_023329017.1	N-formylglutamate deformylase	-
LB481_RS26975 (LB481_26975)	141325..142533	-	1209	WP_023329016.1	imidazolonepropionase	-
LB481_RS26980 (LB481_26980)	142545..143939	-	1395	WP_032738443.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..283677	283677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17255.84 Da
• Isoelectric Point: 7.8840

>T217375 WP_023329018.1 NZ_CP083797:c138733-138251 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAEGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T217375 NZ_CP114180:521517-521867 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT217375 WP_003026799.1 NZ_CP083797:c138987-138721 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT217375 NZ_CP114180:521230-521511 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66