Detailed information of TA system TA217374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 118100..118743
• Replicon: plasmid pINF019_1
• Accession: NZ_CP083797
• Organism: Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LB481_RS26845
• Protein ID: WP_048295728.1
• Coordinates: 118327..118743 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LB481_RS26840
• Protein ID: WP_001261276.1
• Coordinates: 118100..118330 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB481_RS26825 (LB481_26825)	113256..114224	-	969	WP_072193976.1	IS5-like element IS903B family transposase	-
LB481_RS26830 (LB481_26830)	114519..115544	-	1026	WP_001101446.1	IS110 family transposase	-
LB481_RS26835 (LB481_26835)	115776..117737	+	1962	WP_048295727.1	tetratricopeptide repeat protein	-
LB481_RS26840 (LB481_26840)	118100..118330	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LB481_RS26845 (LB481_26845)	118327..118743	+	417	WP_048295728.1	type II toxin-antitoxin system VapC family toxin	Toxin
LB481_RS26850 (LB481_26850)	118913..121051	-	2139	WP_048295729.1	AAA family ATPase	-
LB481_RS28695	121470..121592	-	123	WP_256046161.1	hypothetical protein	-
LB481_RS26855 (LB481_26855)	121692..122042	-	351	WP_167878626.1	hypothetical protein	-
LB481_RS26860 (LB481_26860)	122494..123042	+	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
LB481_RS26865 (LB481_26865)	123089..123523	+	435	WP_001567367.1	cell envelope integrity protein TolA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..283677	283677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15094.58 Da
• Isoelectric Point: 8.5403

>T217374 WP_048295728.1 NZ_CP083797:118327-118743 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T217374 NZ_CP114177:533165-533515 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTAGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT217374 WP_001261276.1 NZ_CP083797:118100-118330 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT217374 NZ_CP114177:532879-533160 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023