Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5252434..5253059 Replicon chromosome
Accession NZ_CP083796
Organism Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LB481_RS25760 Protein ID WP_002882817.1
Coordinates 5252434..5252817 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LB481_RS25765 Protein ID WP_004150355.1
Coordinates 5252817..5253059 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB481_RS25745 (5249800) 5249800..5250702 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LB481_RS25750 (5250699) 5250699..5251334 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LB481_RS25755 (5251331) 5251331..5252260 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LB481_RS25760 (5252434) 5252434..5252817 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LB481_RS25765 (5252817) 5252817..5253059 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LB481_RS25770 (5253264) 5253264..5254181 + 918 WP_023302328.1 alpha/beta hydrolase -
LB481_RS25775 (5254195) 5254195..5255136 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LB481_RS25780 (5255181) 5255181..5255618 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LB481_RS25785 (5255615) 5255615..5256475 - 861 WP_004146232.1 virulence factor BrkB family protein -
LB481_RS25790 (5256469) 5256469..5257068 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T217373 WP_002882817.1 NZ_CP083796:c5252817-5252434 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217373 NZ_CP114176:583544-583903 [Lentibacillus sp. ZS110521]
TTGATTGTGCAAAGAGGCGAAGTTTATTTCGCCGATCTATCTCCTGTTGTCGGATCAGAACAGGGGGGCATCCGCCCGGT
ATTGATCTTGCAAAATGATATTGGTAACAGGTTTAGCCCAACCGTTATCATTGCAGCAATTACCGCACAAATTCAAAAAG
CAAAACTTCCGACTCATGTCGAGATAAACGCAAAACGCTATGGATTTGATCGGAATTCTGTTATCCTGCTTGAACAAATC
AGAACACTTGATAAGCAGCGATTAACTGACAAAATAACAAAGCTGGATAAAGAAATGATGGAAAAGATCAACCATGCATT
GGAAATAAGCCTAGGGCTGAAGGATATGTATGGATCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217373 WP_004150355.1 NZ_CP083796:c5253059-5252817 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217373 NZ_CP114176:583253-583540 [Lentibacillus sp. ZS110521]
TTGTCAGAGAGCTTGCAAGAAGTCTTAGTACGGATACCAAAAAACCTGTTAAATGAGGTGGATGGATTAATGAAGTATGA
AAATAGCGATTTGAGCGATTTCATATGTGAAGCAACCAGAAATTATTTAGAATCCAAGAAAGAGGAGCACATCCAACAAT
TCCGTGATTCCATGATCAAAGGCTACACGGAAATGGCTAGAATTAATCTAACAATCGCTTCAGAAGCATTTCAGGCCGAA
GAGGAAGCAGAAAAGAACACCGTAGAGCGCTCTGTCATCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References