Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4025305..4025924 Replicon chromosome
Accession NZ_CP083796
Organism Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LB481_RS19885 Protein ID WP_002892050.1
Coordinates 4025706..4025924 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LB481_RS19880 Protein ID WP_002892066.1
Coordinates 4025305..4025679 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB481_RS19870 (4020457) 4020457..4021650 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LB481_RS19875 (4021673) 4021673..4024819 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LB481_RS19880 (4025305) 4025305..4025679 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LB481_RS19885 (4025706) 4025706..4025924 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LB481_RS19890 (4026083) 4026083..4026649 + 567 WP_002892030.1 maltose O-acetyltransferase -
LB481_RS19895 (4026621) 4026621..4026761 - 141 WP_004147370.1 hypothetical protein -
LB481_RS19900 (4026782) 4026782..4027252 + 471 WP_002892026.1 YlaC family protein -
LB481_RS19905 (4027227) 4027227..4028678 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
LB481_RS19910 (4028779) 4028779..4029477 + 699 WP_002892021.1 GNAT family protein -
LB481_RS19915 (4029474) 4029474..4029614 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LB481_RS19920 (4029614) 4029614..4029877 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217368 WP_002892050.1 NZ_CP083796:4025706-4025924 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T217368 NZ_CP114168:3668082-3668184 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT217368 WP_002892066.1 NZ_CP083796:4025305-4025679 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT217368 NZ_CP114168:c3668190-3668046 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References