Detailed information of TA system TA021736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 95581..96230
• Replicon: plasmid 1
• Accession: NC_008545
• Organism: Burkholderia cenocepacia HI2424

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BCEN2424_RS33980
• Protein ID: WP_011695140.1
• Coordinates: 95820..96230 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4S3GW89
• Locus tag: BCEN2424_RS33975
• Protein ID: WP_011695139.1
• Coordinates: 95581..95823 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCEN2424_RS33955	90752..91609	-	858	WP_006482109.1	replication initiator protein A	-
BCEN2424_RS33960	92335..93300	-	966	WP_041489699.1	ParB/RepB/Spo0J family partition protein	-
BCEN2424_RS33965	93300..93971	-	672	WP_011695137.1	AAA family ATPase	-
BCEN2424_RS33970	94428..95459	+	1032	WP_011695138.1	non-homologous end-joining DNA ligase	-
BCEN2424_RS33975	95581..95823	+	243	WP_011695139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BCEN2424_RS33980	95820..96230	+	411	WP_011695140.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCEN2424_RS33985	96544..96891	+	348	WP_011695141.1	hypothetical protein	-
BCEN2424_RS37270	96974..97138	+	165	WP_011695142.1	hypothetical protein	-
BCEN2424_RS33990	97324..98046	+	723	WP_011695143.1	helix-turn-helix transcriptional regulator	-
BCEN2424_RS33995	98073..98279	+	207	WP_011695144.1	hypothetical protein	-
BCEN2424_RS34000	98303..98785	+	483	WP_011695145.1	hypothetical protein	-
BCEN2424_RS34005	99099..99440	+	342	WP_041489701.1	hypothetical protein	-
BCEN2424_RS34010	99468..100553	+	1086	WP_011695147.1	hypothetical protein	-
BCEN2424_RS34015	100565..100948	+	384	WP_041489702.1	hypothetical protein	-
BCEN2424_RS34020	100945..101190	+	246	WP_011695149.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164857	164857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.00 Da
• Isoelectric Point: 5.1526

>T21736 WP_011695140.1 NC_008545:95820-96230 [Burkholderia cenocepacia HI2424]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIVVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Nucleotide

• Length: 411 bp

>T21736 NC_008545:95820-96230 [Burkholderia cenocepacia HI2424]
ATGACGCTGTATTTGCTCGACACCAACATACTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACGCGGATTGG
AGAGACCCCGCCGGAGCAAGTGTGCACCTCGATCGTCGTCGCTGCGGAACTGCGATTCGGCGTCTGGAAGCGCGGCTCGT
CAACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGCTGCTAC
GGGCGGCTGCGGGCCGAGCTCGAAAAGCAAGGGCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCTCACGCGCTGGC
CGTCGACGCCGTGCTGGTCACCGACAACACGGCTGAATTTACCCGTGTCGCCGGGCTGCCGGTCGAAAACTGGCTGCGAT
CGGCAACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9290.53 Da
• Isoelectric Point: 4.6856

>AT21736 WP_011695139.1 NC_008545:95581-95823 [Burkholderia cenocepacia HI2424]
MPTHDARHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWAPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT21736 NC_008545:95581-95823 [Burkholderia cenocepacia HI2424]
ATGCCAACCCATGACGCCCGCCACGCGCGCTTGTTTCGCAACGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
GCTGCCGGTCTCGGAGGTCACCATCCACCGGGAAGGCCGCCGCTTGATCATTGAACCCGTGGAAGCGGCACCCTTGCGCG
AGCTGTTCGCCCAATGGGCCCCGCTGGACGAAACCTTTCCGGACATCGAGGACCTTCCGCCTGAGCCGGTGGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A4S3GW89