Detailed information of TA system TA217355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 31520..32430
• Replicon: plasmid pINF352_2
• Accession: NZ_CP083792
• Organism: Klebsiella variicola strain INF352

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LB484_RS28160
• Protein ID: WP_004026354.1
• Coordinates: 31960..32430 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LB484_RS28155
• Protein ID: WP_099745242.1
• Coordinates: 31520..31963 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB484_RS28135 (LB484_28135)	27131..27427	+	297	Protein_41	transposase	-
LB484_RS28140 (LB484_28140)	28154..29641	+	1488	WP_171996283.1	group II intron reverse transcriptase/maturase	-
LB484_RS28145 (LB484_28145)	29726..30148	+	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LB484_RS28150 (LB484_28150)	30148..31419	+	1272	WP_099745241.1	translesion error-prone DNA polymerase V subunit UmuC	-
LB484_RS28155 (LB484_28155)	31520..31963	+	444	WP_099745242.1	DUF2384 domain-containing protein	Antitoxin
LB484_RS28160 (LB484_28160)	31960..32430	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LB484_RS28165 (LB484_28165)	32541..32801	-	261	WP_004026352.1	hypothetical protein	-
LB484_RS29590	33525..33683	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
LB484_RS28180 (LB484_28180)	34062..34304	-	243	WP_086528906.1	hypothetical protein	-
LB484_RS28185 (LB484_28185)	34334..34501	-	168	WP_196241044.1	hypothetical protein	-
LB484_RS28190 (LB484_28190)	34890..35864	+	975	WP_196241043.1	Hok/Gef family protein	-
LB484_RS28195 (LB484_28195)	36062..36439	-	378	WP_099745244.1	phosphonate ABC transporter substrate-binding protein	-
LB484_RS28200 (LB484_28200)	36766..37062	+	297	WP_099745245.1	hydrogenase expression/formation protein HypD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..250854	250854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T217355 WP_004026354.1 NZ_CP083792:31960-32430 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T217355 NZ_CP114165:7596-8012 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT217355 WP_099745242.1 NZ_CP083792:31520-31963 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPAELMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT217355 NZ_CP114165:7369-7599 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0