Detailed information of TA system TA217352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4889638..4890154
• Replicon: chromosome
• Accession: NZ_CP083790
• Organism: Klebsiella variicola strain INF352

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LB484_RS23840
• Protein ID: WP_022065401.1
• Coordinates: 4889638..4889922 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LB484_RS23845
• Protein ID: WP_002886901.1
• Coordinates: 4889912..4890154 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB484_RS23825 (LB484_23825)	4884741..4886396	+	1656	WP_008807132.1	alpha,alpha-phosphotrehalase	-
LB484_RS23830 (LB484_23830)	4886782..4888920	+	2139	WP_032731807.1	anaerobic ribonucleoside-triphosphate reductase	-
LB484_RS23835 (LB484_23835)	4889170..4889634	+	465	WP_022065402.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LB484_RS23840 (LB484_23840)	4889638..4889922	-	285	WP_022065401.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LB484_RS23845 (LB484_23845)	4889912..4890154	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LB484_RS23850 (LB484_23850)	4890232..4892142	-	1911	WP_032756354.1	PRD domain-containing protein	-
LB484_RS23855 (LB484_23855)	4892165..4893319	-	1155	WP_032756352.1	lactonase family protein	-
LB484_RS23860 (LB484_23860)	4893445..4894185	-	741	WP_008807137.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11128.97 Da
• Isoelectric Point: 10.2849

>T217352 WP_022065401.1 NZ_CP083790:c4889922-4889638 [Klebsiella variicola]
MTYELEFDPRAWREWQMPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T217352 NZ_CP114164:4297136-4297354 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT217352 WP_002886901.1 NZ_CP083790:c4890154-4889912 [Klebsiella variicola]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT217352 NZ_CP114164:4296735-4297109 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0