Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4188693..4189312 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LB484_RS20530 Protein ID WP_002892050.1
Coordinates 4189094..4189312 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag LB484_RS20525 Protein ID WP_008805436.1
Coordinates 4188693..4189067 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS20515 (LB484_20515) 4183848..4185041 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LB484_RS20520 (LB484_20520) 4185064..4188210 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
LB484_RS20525 (LB484_20525) 4188693..4189067 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
LB484_RS20530 (LB484_20530) 4189094..4189312 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LB484_RS20535 (LB484_20535) 4189471..4190037 + 567 WP_008805435.1 maltose O-acetyltransferase -
LB484_RS20540 (LB484_20540) 4190009..4190119 - 111 Protein_4029 hypothetical protein -
LB484_RS20545 (LB484_20545) 4190174..4190644 + 471 WP_008805434.1 YlaC family protein -
LB484_RS20550 (LB484_20550) 4190613..4192070 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
LB484_RS20555 (LB484_20555) 4192171..4192869 + 699 WP_023297138.1 GNAT family protein -
LB484_RS20560 (LB484_20560) 4192866..4193006 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LB484_RS20565 (LB484_20565) 4193006..4193269 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217351 WP_002892050.1 NZ_CP083790:4189094-4189312 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T217351 NZ_CP114164:c4167621-4167304 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT217351 WP_008805436.1 NZ_CP083790:4188693-4189067 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT217351 NZ_CP114164:c4167311-4167024 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References