Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1833638..1834228 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag LB484_RS08960 Protein ID WP_008804165.1
Coordinates 1833896..1834228 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag LB484_RS08955 Protein ID WP_012541132.1
Coordinates 1833638..1833895 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS08935 (LB484_08935) 1829247..1829822 + 576 WP_032733307.1 hypothetical protein -
LB484_RS08940 (LB484_08940) 1830000..1830836 + 837 WP_008804155.1 alpha/beta hydrolase -
LB484_RS08945 (LB484_08945) 1831042..1832013 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
LB484_RS08950 (LB484_08950) 1832010..1833110 - 1101 WP_048268588.1 AarF/UbiB family protein -
LB484_RS08955 (LB484_08955) 1833638..1833895 + 258 WP_012541132.1 antitoxin Antitoxin
LB484_RS08960 (LB484_08960) 1833896..1834228 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LB484_RS08975 (LB484_08975) 1834551..1835987 + 1437 WP_049153183.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LB484_RS08985 (LB484_08985) 1836360..1837814 - 1455 WP_008804170.1 AMP nucleosidase -
LB484_RS08990 (LB484_08990) 1837945..1838190 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T217340 WP_008804165.1 NZ_CP083790:1833896-1834228 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T217340 NZ_CP114161:83474-83956 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT217340 WP_012541132.1 NZ_CP083790:1833638-1833895 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT217340 NZ_CP114161:83220-83486 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References