Detailed information of TA system TA021734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1956761..1957987
• Replicon: chromosome
• Accession: NC_008543
• Organism: Burkholderia cenocepacia HI2424

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B1K807
• Locus tag: BCEN2424_RS24475
• Protein ID: WP_011547213.1
• Coordinates: 1957406..1957987 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BCEN2424_RS24470
• Protein ID: WP_011547214.1
• Coordinates: 1956761..1957327 (+)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCEN2424_RS24445	1951827..1952432	-	606	WP_102961848.1	hypothetical protein	-
BCEN2424_RS24450	1952693..1953346	+	654	WP_011547217.1	cysteine hydrolase	-
BCEN2424_RS24455	1953372..1954103	-	732	WP_006498851.1	ribonuclease T2	-
BCEN2424_RS24460	1954231..1954809	-	579	WP_012339630.1	hypothetical protein	-
BCEN2424_RS24465	1954909..1956312	-	1404	WP_011547215.1	hypothetical protein	-
BCEN2424_RS24470	1956761..1957327	+	567	WP_011547214.1	helix-turn-helix domain-containing protein	Antitoxin
BCEN2424_RS24475	1957406..1957987	+	582	WP_011547213.1	HD domain-containing protein	Toxin
BCEN2424_RS24480	1958396..1959589	+	1194	WP_011547212.1	efflux RND transporter periplasmic adaptor subunit	-
BCEN2424_RS24485	1959592..1961637	+	2046	WP_011694806.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-172)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21317.94 Da
• Isoelectric Point: 5.1207

>T21734 WP_011547213.1 NC_008543:1957406-1957987 [Burkholderia cenocepacia HI2424]
MNPDTIHARLAFLREAERLKDVLRSSHTSAGRPESTAEHSWRLCLMALVFTDALPGVDALKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAVRDEIVALWDEYEAAASPEARAAKALDKLETIVQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFRAIREIVDADTQRKIDAGGERA

Nucleotide

• Length: 582 bp

>T21734 NC_008543:1957406-1957987 [Burkholderia cenocepacia HI2424]
ATGAACCCCGATACGATCCACGCGCGACTCGCGTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCAGCCA
CACGTCGGCCGGCCGCCCGGAAAGCACGGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCACCGACGCAC
TGCCCGGCGTCGACGCGCTGAAGCTGCTGAAACTGTGCGTCGTCCACGACCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCCGACAAGAGCGCACATGAACGCGACGACCTGCTGACGCTGACCGCGTCGCTAGACCG
CGCGGTGCGCGACGAGATCGTCGCGCTGTGGGACGAATACGAGGCGGCCGCCTCACCCGAAGCCCGCGCGGCGAAGGCGC
TCGACAAGCTCGAAACGATCGTGCAGCACAACCAGGGCAGCAATCCGCCCGATTTCGACTATGCGTTCAATCTCGGCTAC
GGCCGGCGCTATACCGATGCTGCGCCGCTGTTCCGCGCGATCCGCGAAATCGTCGACGCCGATACGCAGCGCAAGATCGA
CGCGGGTGGGGAGCGCGCATAG

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20565.46 Da
• Isoelectric Point: 10.7076

>AT21734 WP_011547214.1 NC_008543:1956761-1957327 [Burkholderia cenocepacia HI2424]
MPHPPLHPSSLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
SAGRMHLVCDLTHRLLASSPARAAEFSQPLSTLLGTSLWRYATPEIVRMEAALDPFGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRMTLSDSSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT21734 NC_008543:1956761-1957327 [Burkholderia cenocepacia HI2424]
ATGCCCCATCCGCCGCTTCATCCATCCTCGCTCGGCCCGCAACTCAAGCGCTGGCGCGCGCTGCACCGCGTGAAGCAAAG
CCATGCAGCCGAACTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCCTCCAGCAGATGTCGCCCGCCG
AGCGCGCGACGGCCGAACGGCTGCTCGCGGCGCGCCTCGACTCGGCGGGCGACCACGCACTCGCGCGGCTGATCGCCGGC
AGCGCGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGCCTGCTGGCCAGTTCCCCAGCACGCGCGGCCGAGTTCTC
GCAACCGCTTTCGACGTTGCTCGGCACGTCGCTGTGGCGCTACGCGACACCGGAGATCGTCCGCATGGAAGCCGCGCTCG
ACCCGTTCGGCTGGCACGACCGCGCGGGGCCGCCGAGCGTCGAGTTCGACACCGGCGCGAATGCGTCGCGCGTCGTGCCG
ATTCGCGGCAGCCGATGCCGGTGGACACGGATGACACTGTCGGACAGCTCGGCCGTGCGGCTCGTGGAGACGCTCGACGC
ACACTGA

Structures

Toxin

• AlphaFold DB: B1K807