Detailed information of TA system TA217339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1643628..1644625
• Replicon: chromosome
• Accession: NZ_CP083790
• Organism: Klebsiella variicola strain INF352

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LB484_RS08140
• Protein ID: WP_229531768.1
• Coordinates: 1644071..1644625 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MDT5
• Locus tag: LB484_RS08135
• Protein ID: WP_012541033.1
• Coordinates: 1643628..1644074 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB484_RS08120 (LB484_08120)	1639923..1640921	+	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
LB484_RS08125 (LB484_08125)	1641040..1642560	+	1521	WP_008804029.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
LB484_RS08130 (LB484_08130)	1642576..1643586	+	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
LB484_RS08135 (LB484_08135)	1643628..1644074	-	447	WP_012541033.1	helix-turn-helix transcriptional regulator	Antitoxin
LB484_RS08140 (LB484_08140)	1644071..1644625	-	555	WP_229531768.1	HipA domain-containing protein	Toxin
LB484_RS08145 (LB484_08145)	1644654..1645175	-	522	WP_023340544.1	hypothetical protein	-
LB484_RS08150 (LB484_08150)	1645290..1646021	-	732	WP_008804033.1	outer membrane permeability protein SanA	-
LB484_RS08155 (LB484_08155)	1646298..1647182	-	885	WP_012541034.1	cytidine deaminase	-
LB484_RS08160 (LB484_08160)	1647311..1648006	-	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
LB484_RS08165 (LB484_08165)	1647996..1648400	-	405	WP_004201620.1	CidA/LrgA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (2-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20710.03 Da
• Isoelectric Point: 5.7891

>T217339 WP_229531768.1 NZ_CP083790:c1644625-1644071 [Klebsiella variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 555 bp

>T217339 NZ_CP114161:14927-15343 [Klebsiella quasipneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGTCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAATAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16396.05 Da
• Isoelectric Point: 10.5417

>AT217339 WP_012541033.1 NZ_CP083790:c1644074-1643628 [Klebsiella variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT217339 NZ_CP114161:14700-14930 [Klebsiella quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MDT5