Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 774474..775131 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag LB484_RS03845 Protein ID WP_002916310.1
Coordinates 774721..775131 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag LB484_RS03840 Protein ID WP_002916312.1
Coordinates 774474..774740 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS03815 (LB484_03815) 769623..771056 - 1434 WP_032740988.1 6-phospho-beta-glucosidase BglA -
LB484_RS03820 (LB484_03820) 771176..771904 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
LB484_RS03825 (LB484_03825) 771955..772266 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
LB484_RS03830 (LB484_03830) 772430..773089 + 660 WP_008806429.1 hemolysin III family protein -
LB484_RS03835 (LB484_03835) 773245..774228 - 984 WP_008806428.1 tRNA-modifying protein YgfZ -
LB484_RS03840 (LB484_03840) 774474..774740 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
LB484_RS03845 (LB484_03845) 774721..775131 + 411 WP_002916310.1 protein YgfX Toxin
LB484_RS03850 (LB484_03850) 775138..775659 - 522 WP_008806427.1 flavodoxin FldB -
LB484_RS03855 (LB484_03855) 775760..776656 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
LB484_RS03860 (LB484_03860) 776679..777392 + 714 WP_032736403.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LB484_RS03865 (LB484_03865) 777398..779131 + 1734 WP_012540594.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T217338 WP_002916310.1 NZ_CP083790:774721-775131 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T217338 NZ_CP114160:c4821361-4821122 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT217338 WP_002916312.1 NZ_CP083790:774474-774740 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT217338 NZ_CP114160:c4821593-4821351 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References